Monoclonal antibodies OKT 11 and OKT 11A have pan-T reactivity and block sheep erythrocyte "receptors"

European Journal of Immunology
W VerbiG Goldstein

Abstract

Monoclonal antibodies OKT11 (gamma 1) and OKT11A (gamma 2) are described and appear to have similar binding specificities. They bind, in immunofluorescence, with greater than 95% of infant thymocytes, staining both cortical and medullary cells, 65-80% of blood lymphocytes and selectively stain the T cell-dependent paracortical areas of tonsil. A small proportion (9-12%) of bone marrow lymphocytes stain, but this population excludes the terminal transferase-positive cells. Both the gamma 1 and gamma 2 antibodies stain the surface membrane Ig-negative lymphocytes in blood and tonsil and are to block sheep E rosette formation (to normal or leukemic T cells). In contrast, other monoclonal anti-T reagents tested (OKT1, OKT3, OKT4, OKT6, OKT8, OKT9, OKT10) did not block E rosette formation. E rosette formation and OKT11 bindings are coincident on T-ALL cell lines and both are trypsin-sensitive. In a series of 145 leukemias and 26 leukemic cell lines investigated, only leukemias with a T cell phenotype including E rosette positivity were reactive with OKT11 and OKT11A. OKT11A binds to a polypeptide of approximately 50 000 molecular weight on thymic lymphocytes. This structure may carry the recognition site for sheep erythrocytes. Thes...Continue Reading

References

Jun 1, 1978·Journal of the National Cancer Institute·J MinowadaE Tatsumi
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R LevyR Warnke
Mar 1, 1979·European Journal of Immunology·A J McMichaelC Milstein
Apr 1, 1966·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R C Graham, M J Karnovsky
Apr 1, 1980·The Journal of Experimental Medicine·E L ReinherzS F Schlossman
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·E L ReinherzS F Schlossman
Jan 1, 1981·The Journal of Experimental Medicine·M KamounR C Nowinski

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Citations

Jan 1, 1997·Journal of Clinical Laboratory Analysis·G NuñezN F Mendes
Aug 1, 1990·Journal of Biomedical Materials Research·I BravoM de Sousa
Jan 1, 1991·Journal of Clinical Laboratory Analysis·E N ItanoN F Mendes
Jan 1, 1991·Journal of Clinical Laboratory Analysis·E N ItanoN F Mendes
Mar 1, 1985·Journal of Clinical Immunology·H Tsuda, K Takatsuki
Oct 1, 1982·Journal of Clinical Immunology·G SemenzatoG Tridente
Jan 1, 1986·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·P YouinouC Ferec
Jul 24, 1986·Journal of Immunological Methods·S S Tabibzadeh, M A Gerber
Nov 1, 1983·Mechanisms of Ageing and Development·D BroheeP Neve
Jan 1, 1984·Developmental and Comparative Immunology·D A Higgins
Sep 1, 1983·Journal of Reproductive Immunology·H ZolaP C Beverley
Oct 1, 1984·Veterinary Immunology and Immunopathology·S T Licence, R M Binns
May 1, 1985·Veterinary Immunology and Immunopathology·H A LewinD Bernoco
Feb 1, 1984·Human Immunology·C Rohowsky-KochanN Suciu-Foca
Dec 1, 1991·Immunology and Cell Biology·S A IshijimaT Suzuta
Mar 29, 2002·Immunology·Masayuki ShimojimaYukinobu Tohya
Nov 11, 1982·The New England Journal of Medicine·J CossmanS J Korsmeyer
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·W A SewellM J Crumpton
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B Seed, A Aruffo
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·Y TakaiS H Herrmann
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P H SayreE L Reinherz
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·N E RichardsonE L Reinherz
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·B E BiererS J Burakoff
Dec 10, 2002·Cancer Biotherapy & Radiopharmaceuticals·S SchuhknechtJ Atzpodien
Jan 1, 1985·Hybridoma·E De Nardin, N Tanigaki
Jan 1, 1984·Journal of Interferon Research·R C FuhlbriggeE C Borden
Feb 1, 1990·Scandinavian Journal of Immunology·A J PotocnikR A Kroczek
Jun 1, 1994·British Journal of Pharmacology·D A WalshJ Wharton
Sep 1, 1987·Annals of the Rheumatic Diseases·M Segerberg-KonttinenY T Konttinen

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