PMID: 7544051Aug 20, 1995Paper

Monoclonal antibodies raised against covalently crosslinked complexes of human immunodeficiency virus type 1 gp120 and CD4 receptor identify a novel complex-dependent epitope on gp 120

Virology
A L DeVicoR Pal

Abstract

The binding of the human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein, gp120, to its cell surface receptor, CD4, represents a molecular interaction involving distinct alterations in protein structure. Consequently, the pattern of epitopes presented on the gp120-CD4 complex should differ from those on free gp120. To investigate this concept, mice were immunized with covalently crosslinked complexes of viral HIV-1IIIBgp120 and soluble CD4. Two monoclonal antibodies (MoAbs) obtained from the immunized mice exhibited a novel epitope specificity. The MoAbs were marginally reactive with HIV-1IIIBgp120, highly reactive with gp120-CD4 complexes, and unreactive with soluble CD4. The same pattern of reactivity was seen in solid-phase assays using HIV-1(451)gp120. A similar specificity for complexes was evident in flow cytometry experiments, in which MoAb reactivity was dependent upon the attachment of gp120 to CD4-positive cells. In addition, MoAb reactivity was detected upon the interaction of CD4 receptors with purified HIV-1IIIB virions. Notably, seroantibodies from HIV-positive individuals competed for MoAb binding, indicating that the epitope is immunogenic in humans. The results demonstrated that crosslinked gp120-CD...Continue Reading

Citations

Dec 7, 2013·Retrovirology·Anna Roitburd-BermanJonathan M Gershoni
Dec 19, 2013·Antiviral Research·Luis Menéndez-Arias, Mar Alvarez
Oct 25, 2007·Proceedings of the National Academy of Sciences of the United States of America·Anthony DeVicoRanajit Pal
Aug 23, 2002·Proceedings of the National Academy of Sciences of the United States of America·Timothy FoutsRanajit Pal
May 17, 2006·Microbes and Infection·Donato ZipetoUmberto Bertazzoni
Aug 3, 2004·Expert Review of Vaccines·Indresh K SrivastavaSusan W Barnett
Apr 19, 2006·Gastroenterology·Penelope D OttewellD Mark Pritchard

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