PMID: 6408643Jul 1, 1983Paper

Monoclonal antibodies specific for Hantaan virus

Proceedings of the National Academy of Sciences of the United States of America
M C FrankoD C Gajdusek

Abstract

Six hybridoma cell line producing monoclonal antibodies to Hantaan virus were established by fusion of NS-1 mouse myeloma cells with spleen cells of mice immunized with Hantaan virus strain 76-118. The specificity of these monoclonal antibodies was established by immunoblotting analysis and immunofluorescence. Five of the clones reacted with antigens on the cell surface and in the cytoplasm, and one clone reacted with a determinant expressed only in the cytoplasm of the infected cells. Two of the clones produced antibodies that reacted with a Mr 50,000 polypeptide in virus-infected cellular extracts and purified virus preparations. The monoclonal antibodies were used to examine the antigenic relationship among Hantaan virus strains and between Hantaan virus and Prospect Hill virus and the virus of nephropathia epidemica. Three antibodies were capable of distinguishing between the Lee strain and the 760-118 strain of Hantaan virus and three additional antibodies reacted with determinants shared by both virus strains. None of the six reacted with Prospect Hill virus or the virus of nephropathia epidemica.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Apr 1, 1976·European Journal of Immunology·G KöhlerC Milstein
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M C FrankoD C Gajdusek
Aug 1, 1982·Virology·J F Smith, D Y Pifat
Sep 1, 1980·Proceedings of the Society for Experimental Biology and Medicine·F H Pruslin, W F Scherer

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Citations

Jan 1, 1991·Archives of Virology·S L RuoS P Fisher-Hoch
Jul 26, 2012·Archives of Virology·Mathias SchlegelBernd Köllner
Oct 20, 2009·Vaccine·Connie Schmaljohn
Jul 17, 2020·MSphere·Taylor B Engdahl, James E Crowe
Jul 1, 1995·Clinical and Diagnostic Laboratory Immunology·I S HaJ S Lee
Aug 6, 2009·Journal of Virological Methods·Giovanny A C A MazzarottoClaudia N Duarte dos Santos

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