PMID: 2497510Mar 1, 1989Paper

Monoclonal antibodies specific for variable and constant domains of murine lambda chains

Scandinavian Journal of Immunology
B Bogen

Abstract

Rat monoclonal antibodies directed against the BALB/c myeloma protein M315 (alpha,lambda 2) are described. 9A8 (IgG1) binds the V domain of lambda 2 and cross-reacts with lambda 1 and lambda 3 chains. 2B6 (IgG2a) is directed to the C domain of lambda 2 and cross-reacts with C lambda 3. The antibodies bind isolated chains as well as complete immunoglobulins. The monoclonals detect soluble immunoglobulin (radioimmunoassay), immunoglobulin immobilized on polystyrene (enzyme-linked immunosorbent assay), immunoglobulin bound to nitrocellulose (immunoblotting), and surface immunoglobulin intercalated in cell membranes (immunofluorescence). The antibodies are easily purified on protein G immunosorbents and may be biotinylated or conjugated with fluorescein isothiocyanate without loss of capacity to bind. In addition to the anti-lambda antibodies, a C alpha 2/C alpha 3-specific monoclonal antibody, 8D2 (IgG2a) is described.

References

Nov 1, 1978·The Journal of Experimental Medicine·T Cotner, H N Eisen
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S TonegawaW Gilbert
Feb 28, 1978·Biochemical and Biophysical Research Communications·P J Fraker, J C Speck
May 1, 1987·European Journal of Immunology·R DildropK Rajewsky
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P SanchezP A Cazenave
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T AzumaH N Eisen
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J MillerU Storb
Jul 22, 1982·Nature·A L BothwellD Baltimore
Jan 1, 1983·Hybridoma·S WeissM Cohn
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·B BlombergS Tonegawa
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·B Blomberg, S Tonegawa
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·E SelsingU Storb

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Citations

Feb 8, 2005·Cancer Immunology, Immunotherapy : CII·Katrin U LundinBjarne Bogen
Jul 1, 1991·Molecular Immunology·A J GeorgeF K Stevenson
Jun 1, 1991·Scandinavian Journal of Immunology·G F Lauritzsen, B Bogen
Jul 20, 2007·The Cochrane Database of Systematic Reviews·M E J L HulscherR Grol

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