PMID: 3319376Oct 1, 1987Paper

Monoclonal antibodies to bovine collagenase inhibitor

Collagen and Related Research
S KodamaT Hayakawa

Abstract

Hybridoma antibodies against bovine collagenase inhibitor were produced by fusion of myeloma cells NS-1 (P3-NS1-1) with spleen cells from mice hyperimmunized with collagenase inhibitor purified from the explant medium of bovine dental pulps. Hybridomas positive by an enzyme-linked immunosorbent assay (ELISA) for bovine collagenase inhibitor were cloned by the dilution method. Seventeen hybridomas producing antibodies were isolated, four of which also recognized purified human collagenase inhibitor in the ELISA. Using a monoclonal antibody-Sepharose affinity column, we easily purified both bovine and human collagenase inhibitors to homogeneity. They showed the same mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, corresponding to a molecular mass of 32,000 daltons.

References

May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T W CooperH G Welgus
Jul 1, 1985·The Journal of Clinical Investigation·H G WelgusS L Teitelbaum
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D F CarmichaelG P Stricklin
Oct 1, 1986·Collagen and Related Research·T HoshinoT Hayakawa
Nov 15, 1973·Journal of Molecular Biology·U K Laemmli, M Favre
Nov 15, 1984·European Journal of Biochemistry·K OtsukaH Limeback
Dec 1, 1984·Collagen and Related Research·E D HarrisS M Krane
Jan 1, 1981·Journal of Periodontal Research·S B Geiger, E Harper
Jun 16, 1981·Biochemical and Biophysical Research Communications·J AggelerZ Werb
May 1, 1980·Analytical Biochemistry·S I RennardP G Robey

❮ Previous
Next ❯

Citations

Jan 5, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T SobueT Hayakawa
Feb 13, 2010·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Masaki ShimizuTsutomu Kobayashi
Jun 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J LiC S Lieber
Jan 2, 1994·International Journal of Cancer. Journal International Du Cancer·Y TsuchiyaM Seiki
Jan 31, 1996·Clinica Chimica Acta; International Journal of Clinical Chemistry·E OhuchiY Okada
Oct 15, 1993·Clinica Chimica Acta; International Journal of Clinical Chemistry·J ZhangT Hayakawa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.