PMID: 7542251Jul 1, 1995Paper

Monoclonal antibodies to CD44 and their influence on hyaluronan recognition

The Journal of Cell Biology
Z ZhengP W Kincade

Abstract

Antibodies to CD44 have been used to inhibit a variety of processes which include lymphohemopoiesis, lymphocyte migration, and tumor metastasis. Some, but not all, CD44-mediated functions derive from its ability to serve as a receptor for hyaluronan (HA). However, sites on CD44 that interact with either ligands or antibodies are poorly understood. Interspecies rat/mouse CD44 chimeras were used to analyze the specificity of 25 mAbs and to determine that they recognize at least seven epitopes. Amino acid substitutions that resulted in loss of antibody recognition were all located in the region of homology to other cartilage link family proteins. While at least five epitopes were eliminated by single amino acid replacements, multiple residues had to be changed to destroy binding by other antibodies. One antibody was sensitive to changes in any of three separate parts of the molecule and some antibodies to distinct epitopes cross-blocked each other. Certain antibodies had the ability to increase HA binding by lymphocytes but this did not correlate absolutely with antibody specificity and was only partially attributable to CD44 cross-linking. Antibodies that consistently blocked HA recognition were all sensitive to amino acid change...Continue Reading

References

Aug 1, 1992·The Journal of Cell Biology·L ThomasI Stamenkovic
Oct 1, 1992·European Journal of Immunology·J Lesley, R Hyman
Sep 11, 1990·Nucleic Acids Research·S Mizushima, S Nagata
Aug 1, 1990·Immunology Today·C G FigdorG D Keizer
Jul 1, 1990·The Journal of Experimental Medicine·K MiyakeP W Kincade
Feb 1, 1990·The Journal of Experimental Medicine·K MiyakeP W Kincade
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·C NottenburgT St John
Jan 1, 1986·Immunogenetics·C M IsackeI S Trowbridge
Mar 1, 1982·Immunogenetics·J Lesley, I S Trowbridge
Apr 1, 1994·International Immunology·N Toyama-Sorimachi, M Miyasaka
Feb 1, 1995·European Journal of Immunology·A PerschlR Hyman
Sep 1, 1993·The Journal of Cell Biology·D T ShihC A Buck
Jul 1, 1993·The Journal of Cell Biology·R J PeachA Aruffo
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·W KnudsonC B Knudson

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Citations

Jan 5, 2002·Arthritis and Rheumatism·R StoopK Mikecz
Jan 25, 2002·International Journal of Cancer. Journal International Du Cancer·Mehdi Rahmanian, Paraskevi Heldin
Oct 25, 2007·Brain Cell Biology·Marzenna WiranowskaPaul E Gottschall
May 13, 1999·Progress in Histochemistry and Cytochemistry·K Ostergaard, D M Salter
Oct 1, 1996·Current Opinion in Cell Biology·D DunonB A Imhof
Oct 23, 1997·Current Opinion in Cell Biology·P W KincadeL Hanson
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·A Varki
Aug 20, 2002·Journal of Hematotherapy & Stem Cell Research·Andrés HidalgoJoaquin Teixidó
May 20, 2009·Glycobiology·Evisabel A CraigTodd D Camenisch
Oct 24, 2000·Kidney International·K TakazoeD J Nikolic-Paterson
Jun 24, 2003·Infection and Immunity·Emma ErikssonMartin E Rottenberg
Apr 1, 1997·Molecular Pathology : MP·Z Rudzki, S Jothy
Apr 21, 2007·American Journal of Respiratory and Critical Care Medicine·Shigeki KatohMitsuomi Hirashima
Oct 18, 2000·The Journal of Clinical Investigation·C CywesM R Wessels
Dec 1, 2006·The Journal of Reproduction and Development·Masaki YokooEimei Sato
Aug 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J R KaniaS R Kupfer
Jun 4, 2014·Journal of Dermatological Science·Pi-Ling ChangKang-Jey Ho
Dec 17, 2009·Expert Opinion on Therapeutic Targets·Satoshi Tanaka
Jul 1, 1996·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·K J NoonanR J Midura
Mar 10, 2009·Biochemical and Biophysical Research Communications·Woro Anindito Sri TunjungEimei Sato
Jan 27, 2009·Experimental and Molecular Pathology·Alaa AfifyLaura Nguyen
Dec 4, 2009·International Journal of Cancer. Journal International Du Cancer·Marzenna WiranowskaAnna Plaas
May 17, 2003·The Journal of Clinical Investigation·Shigeki KatohShigeru Matsukura
Apr 22, 2015·Cell·Luca G GuidottiMatteo Iannacone
Jul 31, 1998·Experimental Cell Research·V LadedaE Bal de Kier Joffé

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