PMID: 9557640Apr 29, 1998Paper

Monoclonal antibodies to distinct sites on herpes simplex virus (HSV) glycoprotein D block HSV binding to HVEM

Journal of Virology
Anthony V NicolaG H Cohen

Abstract

HVEM (for herpesvirus entry mediator) is a member of the tumor necrosis factor receptor superfamily and mediates entry of many strains of herpes simplex virus (HSV) into normally nonpermissive Chinese hamster ovary (CHO) cells. We used sucrose density centrifugation to demonstrate that purified HSV-1 KOS virions bind directly to a soluble, truncated form of HVEM (HVEMt) in the absence of any other cell-associated components. Therefore, HVEM mediates HSV entry by serving as a receptor for the virus. We previously showed that soluble, truncated forms of HSV glycoprotein D (gDt) bind to HVEMt in vitro. Here we show that antibodies specific for gD, but not the other entry glycoproteins gB, gC, or the gH/gL complex, completely block HSV binding to HVEM. Thus, virion gD is the principal mediator of HSV binding to HVEM. To map sites on virion gD which are necessary for its interaction with HVEM, we preincubated virions with gD-specific monoclonal antibodies (MAbs). MAbs that recognize antigenic sites Ib and VII of gD were the only MAbs which blocked the HSV-HVEM interaction. MAbs from these two groups failed to coprecipitate HVEMt in the presence of soluble gDt, whereas the other anti-gD MAbs coprecipitated HVEMt and gDt. Previous map...Continue Reading

References

Mar 1, 1992·The Journal of Cell Biology·M T ShiehP G Spear
Jan 1, 1992·Advances in Experimental Medicine and Biology·G H CohenR J Eisenberg
May 1, 1989·Journal of Virology·V J IsolaG H Cohen
Feb 1, 1985·Journal of Virology·R J EisenbergG H Cohen
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A O Fuller, P G Spear
May 1, 1980·Journal of Virology·G H CohenR J Eisenberg
Aug 1, 1980·Infection and Immunity·L PereiraJ R Baringer
Sep 1, 1996·Journal of Virology·C G HandlerG H Cohen
May 23, 1997·The Journal of Biological Chemistry·H HsuW J Boyle

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Citations

Feb 27, 2001·Journal of Virology·H BultmannC R Brandt
Feb 8, 2003·DNA and Cell Biology·Edward A BurtonJoseph C Glorioso
May 28, 2003·Journal of Virology·Tina M CairnsRoselyn J Eisenberg
May 5, 2004·Proceedings of the National Academy of Sciences of the United States of America·Francesca CocchiGabriella Campadelli-Fiume
Aug 2, 2008·Cellular Microbiology·Maria Teresa SciortinoAntonio Mastino
Jul 4, 2012·Viruses·Roselyn J EisenbergGary H Cohen
Feb 27, 2013·Reviews in Medical Virology·Thessicar E AntoineDeepak Shukla
Dec 23, 2004·Journal of Virology·Sarah A ConnollyRoselyn J Eisenberg
Oct 30, 2004·Current Eye Research·David H MilesSreedharan Athmanathan
Apr 5, 2007·Annals of the New York Academy of Sciences·M Teresa SciortinoAntonio Mastino
Feb 17, 2009·Human Gene Therapy·Deborah A Ryan, Howard J Federoff
Oct 9, 2013·Acta Crystallographica. Section D, Biological Crystallography·Cheng-Chung LeeAndrew H-J Wang
Jul 18, 2002·Oral Microbiology and Immunology·S-L HungY-T Chen
Jun 29, 2004·Journal of Virology·Anthony V Nicola, Stephen E Straus
Feb 12, 2002·Journal of Virology·Claude KrummenacherRoselyn J Eisenberg

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