Abstract
We report on the development of monoclonal antibodies directed against the transmembrane portion of the envelope of HTLV-III451 gp41. One of these monoclonal antibodies, designated M71/2B4, was found to cross-react with transmembrane proteins from other independent isolates of HIV-1, namely IIIB, MN, and RF. Thus, this monoclonal antibody identifies an epitope located in a region of gp41 that is conserved among all these isolates. To identify this conserved region a series of E. coli recombinant proteins were screened in immunoblot with M71/2B4. From these results the epitope recognized by this antibody appears to map at the amino terminus of gp41, in the region indicated between the cleavage site with gp120 (aa 508) and the HindIII site (aa647).
References
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jun 6, 1986·Cell·B R StarcichR C Gallo
Jul 4, 1986·Cell·M AlizonP Sonigo
Feb 1, 1987·Journal of Virology·S ModrowH Wolf
Jun 1, 1987·Journal of Virology·D D HoV L Sato
Feb 1, 1985·Science·R Sanchez-PescadorJ A Levy
Jan 24, 1985·Nature·L RatnerK Baumeister
Jan 1, 1985·Cell·S Wain-HobsonM Alizon
May 31, 1985·Science·J S AllanM Essex
Sep 27, 1985·Science·F D VeroneseM G Sarngadharan
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S G DevareV S Kalyanaraman
Jan 1, 1987·Gene·A SrinivasanR Sanchez-Pescador
Oct 1, 1988·AIDS Research and Human Retroviruses·V S KalyanaramanM G Sarngadharan
Jun 1, 1988·Virology·C GurgoM S Reitz
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S M DesaiS G Devare
Jan 1, 1983·Methods in Enzymology·B P Nichols, C Yanofsky
Jul 1, 1984·The Journal of Investigative Dermatology·R J WatsonL W Enquist
Sep 30, 1982·Journal of Immunological Methods·C L Reading
Citations
Jun 24, 1991·Journal of Immunological Methods·D L MatourZ L Jonak
Nov 1, 1989·Virology·F ChiodiE M Fenyö
Mar 1, 1990·AIDS Research and Human Retroviruses·V S KalyanaramanM G Sarngadharan
Jul 1, 1990·Journal of Virology·R W DomsB Moss