Monoclonal Antibodies to Intracellular Stages of Cryptosporidium parvum Define Life Cycle Progression In Vitro

MSphere
Georgia WilkeL David Sibley

Abstract

Among the obstacles hindering Cryptosporidium research is the lack of an in vitro culture system that supports complete life development and propagation. This major barrier has led to a shortage of widely available anti-Cryptosporidium antibodies and a lack of markers for staging developmental progression. Previously developed antibodies against Cryptosporidium were raised against extracellular stages or recombinant proteins, leading to antibodies with limited reactivity across the parasite life cycle. Here we sought to create antibodies that recognize novel epitopes that could be used to define intracellular development. We identified a mouse epithelial cell line that supported C. parvum growth, enabling immunization of mice with infected cells to create a bank of monoclonal antibodies (MAbs) against intracellular parasite stages while avoiding the development of host-specific antibodies. From this bank, we identified 12 antibodies with a range of reactivities across the parasite life cycle. Importantly, we identified specific MAbs that can distinguish different life cycle stages, such as trophozoites, merozoites, type I versus II meronts, and macrogamonts. These MAbs provide valuable tools for the Cryptosporidium research com...Continue Reading

References

Apr 1, 1992·Veterinary Parasitology·F MartinezA Osuna
Aug 1, 1990·Antimicrobial Agents and Chemotherapy·V McDonaldK P McAdam
Jan 1, 1986·Pediatric Infectious Disease·C R Sterling, M J Arrowood
Feb 1, 1986·The Journal of protozoology·W L Current, N C Reese
Nov 1, 1996·Digestive Diseases and Sciences·P D GreenbergJ P Cello
Apr 29, 1998·Advances in Parasitology·S Tzipori, J K Griffiths
Jan 12, 1999·Microbiology·Laurence TetleyGraham H Coombs
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·M E PerkinsS M Le Blancq
Sep 6, 2000·Clinical and Diagnostic Laboratory Immunology·C WeirK Williams
Aug 14, 2001·Infection and Immunity·D A ElliottD P Clark
Mar 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Naomi S Morrissette, L David Sibley
Jul 5, 2002·Clinical Microbiology Reviews·Michael J Arrowood
Mar 27, 2004·Science·Mitchell S AbrahamsenVivek Kapur
Oct 29, 2004·Nature·Ping XuGregory A Buck
Nov 5, 2004·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Franz Petry
Aug 28, 2007·Molecular and Biochemical Parasitology·Roberta M O'ConnorHonorine D Ward
Oct 2, 2007·The Journal of Biological Chemistry·Najma BhatHonorine D Ward
Dec 17, 2010·AIDS·Roberta M O'connorHonorine D Ward
Oct 28, 2011·Revista Da Sociedade Brasileira De Medicina Tropical·Silvia Cristina OsakiVanete Thomaz Soccol
May 15, 2012·Lancet·Li LiuUNKNOWN Child Health Epidemiology Reference Group of WHO and UNICEF
Jan 9, 2013·Clinical Microbiology Reviews·Maha BouzidKevin M Tyler
Aug 27, 2013·International Journal for Parasitology·Jan Šlapeta
Nov 16, 2013·Nature Protocols·Hiroyuki Miyoshi, Thaddeus S Stappenbeck
Nov 16, 2013·Nature·Boris Striepen
Dec 25, 2013·Antimicrobial Agents and Chemotherapy·Suresh Kumar GorlaLizbeth Hedstrom
Sep 19, 2014·PLoS Pathogens·Daniel BargieriRobert Ménard
Jul 16, 2015·Nature·Sumiti VinayakBoris Striepen

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Citations

Sep 8, 2020·Expert Opinion on Drug Discovery·Melissa S Love, Case W McNamara
Sep 20, 2018·F1000Research·Seema BhalchandraHonorine D Ward
Feb 16, 2021·Current Clinical Microbiology Reports·Derek J Pinto, Sumiti Vinayak
Dec 15, 2020·Frontiers in Cellular and Infection Microbiology·N Bishara Marzook, Adam Sateriale
Oct 9, 2020·Cell Host & Microbe·Amandine Guérin, Boris Striepen
Oct 6, 2021·MBio·Nicolas Dos Santos PachecoDominique Soldati-Favre

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Methods Mentioned

BETA
IECs
PCR
confocal microscopy
electron microscopy
PCRs
reverse transcription-PCR

Software Mentioned

AxioVision
Volocity
AMT Image Capture Engine
ZEN

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