Monoclonal antibodies to serotype 2 and serotype 15 outer membrane proteins of Neisseria meningitidis and their use in serotyping.

Infection and Immunity
Wendell D ZollingerB Brandt

Abstract

A series of murine monoclonal antibodies to serotype 2 and serotype 15 strains of Neisseria meningitidis were produced which were specific for outer membrane proteins of classes 1, 2, 3, and 5. A panel of eight monoclonal antibodies that exhibited a high degree of serotype specificity when reacted with prototype strains of known serotype were selected for study. Each of the corresponding epitopes was localized on a specific outer membrane protein by means of immunoprecipitation, electroblotting, or both. The serotype 2a-, 2b-, and 2c-specific antibodies bound to the class 2 protein, the serotype 15-specific antibody bound to the class 3 protein, two antibodies (3-1-P1.2 and 3-1-P1.16) bound to class 1 proteins, and two antibodies (1-1-P5.1 and 3-1-P5.2) bound to class 5 proteins. Six of these monoclonal antibodies were used in a spot-blot procedure to survey 122 case isolates (groups B, C, Y, and W135) and 363 carrier isolates (all serogroups) for the presence of the 2a, 2b, 2c, 15, P1.2, and P1.16 epitopes. A total of 66% of the case isolates and 30% of the carrier isolates reacted with one or more of the monoclonal antibodies. The use of monoclonal antibodies for serotyping of meningococci appears to be feasible and easy and ...Continue Reading

References

May 1, 1979·The Journal of Clinical Investigation·W D ZollingerS Berman
May 1, 1977·Infection and Immunity·R E Mandrell, W D Zollinger
Jul 1, 1974·The Journal of Experimental Medicine·C E Frasch, E C Gotschlich
Jan 1, 1982·Analytical Biochemistry·R HawkesJ Gordon

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Citations

Aug 1, 1990·Epidemiology and Infection·C CruzI Canepa
Aug 1, 1985·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology·O G Brakstad, J A Maeland
Jan 1, 1987·Antonie van Leeuwenhoek·W D ZollingerB L Brandt
Jun 1, 1991·Molecular Microbiology·E L AhoJ G Cannon
Dec 1, 1985·European Journal of Clinical Microbiology·C E Frasch
Dec 1, 1988·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·P CalainB Hirschel
Sep 1, 1996·FEMS Immunology and Medical Microbiology·M G DanelliC E Frasch
Jul 3, 1999·Clinical and Diagnostic Laboratory Immunology·E WedegeW D Zollinger
May 1, 1992·Journal of Clinical Microbiology·C T SacchiC E Melles
Jul 1, 1992·Infection and Immunity·H K GuttormsenC O Solberg
Apr 1, 1988·Infection and Immunity·A K BhattacharjeeW D Zollinger
Jul 1, 1989·Journal of Clinical Microbiology·J M Libby, H G Wada

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