PMID: 6168775Aug 1, 1981Paper

Monoclonal antibodies to two glycoproteins of herpes simplex virus type 2

Journal of Virology
N BalachandranW E Rawls

Abstract

Monoclonal antibodies to herpes simplex virus type 2 were found to precipitate different numbers of radiolabeled polypeptides from lysates of virus-infected cells. Antibodies directed against two viral glycoproteins were characterized. Antibodies from hybridoma 17 alpha A2 precipitated a 60,000-molecular-weight polypeptide which chased into a 66,000- and 79,000-molecular-weight polypeptide. All three polypeptides labeled in the presence of [3H]glucosamine and had similar tryptic digest maps. The 60,000-molecular-weight polypeptide also chased into a 31,000-molecular-weight species which did not label with [3H]glucosamine. Antibodies from hybridoma 17 beta C2 precipitated a 50,000-molecular-weight polypeptide which chased into a 56,000- and 80,000-molecular weight polypeptide. These polypeptides also shared a similar tryptic digest map and labeled with [3H]glucosamine. Both monoclonal antibodies were herpes simplex virus type 2 specific. The viral proteins precipitated by 17 alpha A2 antibodies had characteristics similar to those reported for glycoprotein E, whereas the proteins precipitated by 17 beta C2 antibodies appeared to represent a glycoprotein not previously described. This glycoprotein should be tentatively designated...Continue Reading

References

Jan 1, 1974·Intervirology·P SethJ L Melnick
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
May 1, 1980·The Journal of General Virology·I W Halliburton
Aug 1, 1980·Infection and Immunity·L PereiraJ R Baringer
Mar 1, 1981·Journal of Virological Methods·R A KillingtonS Bacchetti

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Citations

Jun 1, 1988·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·M Ackermann
May 1, 1994·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·L Haarr, S Skulstad
Apr 1, 1986·Journal of Virology·M W Wathen, L M Wathen
May 1, 1986·Journal of Virology·R V SrinivasR W Compans
Jun 1, 1985·Journal of Virology·N Balachandran, L M Hutt-Fletcher
Nov 1, 1986·Journal of Virology·N BalachandranL M Hutt-Fletcher
Jul 1, 1982·Journal of Clinical Microbiology·N BalachandranW E Rawls
Apr 1, 1983·Infection and Immunity·C GroseW L McGuire
Oct 1, 1982·Journal of Virology·N BalachandranS Bacchetti
Aug 1, 1985·Archives of Biochemistry and Biophysics·F Serafini-CessiG Campadelli-Fiume

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