PMID: 8996707Dec 1, 1996Paper

Monoclonal antibody against bovine Lactoferricin and its epitopic site

The Journal of Veterinary Medical Science
K ShimazakiN Suzuki

Abstract

Bovine lactoferricin (LFcin B) is a strong antimicrobial peptide derived from N-lobe of lactoferrin. To study the immunochemical and structural properties of LFcin B, monoclonal antibody (mAb) was prepared and the amino acid sequence concerning with the binding to mAb has been identified. Mice injected with LFcin B showed no production of antibody specific to this peptide, whereas those with LFcin B-KLH conjugate produced anti-LFcin B antibodies. None of the mAb reacted with bovine lactoferrin C-lobe, human lactoferrin or LFcin H. By the reactivity of the mAb against the peptides synthesized on cellulose membranes using SPOTs and against chemically modified derivatives of LFcin B, the antigenic determinant of LFcin B was identified to be the sequence of "QWR".

Citations

Mar 14, 2012·FEMS Immunology and Medical Microbiology·Deirdre Fitzgerald-HughesHilary Humphreys
Aug 8, 2006·The Journal of Veterinary Medical Science·Yumiko KomineKen-ichi Komine
Jul 25, 2006·Expert Opinion on Investigational Drugs·Jamie S Mader, David W Hoskin

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