Monoclonal antibody-based enzyme linked immunosorbent assay for the analysis of jasmonates in plants

Journal of Integrative Plant Biology
Ai-Xing DengQing X Li

Abstract

Methyl jasmonate (MeJA) and its free-acid form, jasmonic acid (JA) are naturally occurring plant growth regulators widely distributed in higher plants. In order to improve the sensitivity for the analysis of MeJA at low levels in small amounts of plant samples, a monoclonal antibody (MAb) (designated as MAb 3E(5)D(7)C(4)B(6)) against MeJA was derived from a JA-bovine serum albumin (BSA) conjugate as an immunogen. The antibody belongs to the IgG(1) subclass with a kappa type light chain and has a dissociation constant of approximately 6.07 x 10(-9) M. MAb3E(5)D(7)C(4)B(6) is very specific to MeJA. It was used to develop a direct competitive enzyme-linked immunosorbent assay (dcELISA), conventional and simplified indirect competitive ELISAs (icELISA). JA was derivatized into MeJA for the ELISA analysis. The IC(50) value and detection range for MeJA were, respectively, 34 and 4-257 ng/mL by the conventional icELISA, 21 and 3-226 ng/mL by the simplified icELISA and 5.0 and 0.7-97.0 ng/mL by the dcELISA. The dcELISA was more sensitive than either the conventional or simplified icELISA. The assays were used to measure the content of jasmonates as MeJA in tobacco leaves under drought stress or inoculated with tobacco mosaic virus and ...Continue Reading

References

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Citations

Jan 5, 2012·Analytical and Bioanalytical Chemistry·Fuyou DuHuwei Liu
Dec 4, 2009·Nucleic Acids Research·Yusuke MiyachiAkihiko Kondo
Mar 23, 2011·Plant Science : an International Journal of Experimental Plant Biology·Kun WuZheng-Qiang Ma
Jun 16, 2009·Journal of Integrative Plant Biology·Hao-Ru YangWei-Dong Huang
Jul 12, 2017·Journal of Agricultural and Food Chemistry·Liu WangLin Shen
Mar 27, 2019·Journal of Integrative Plant Biology·Gui Jie LeiShao Jian Zheng
Aug 11, 2018·Journal of Agricultural and Food Chemistry·Rui LiLin Shen

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