Abstract
Three monoclonal antibodies (MAbs) to lipopolysaccharide of Actinobacillus actinomycetemcomitans strain Y4 (serotype b) and eight MAbs to a serotype b-specific polysaccharide antigen of strain Y4 were obtained. Latex particles sensitized with an MAb to the Y4 lipopolysaccharide produced a positive agglutination with whole cells of all three serotypes of A. actinomycetemcomitans, but not with Haemophilus aphrophilus, Haemophilus paraphrophilus, Haemophilus influenzae, Porphyromonas (Bacteroides) gingivalis, "Bacteroides" intermedius, Fusobacterium nucleatum and Escherichia coli. On the other hand, latex particles sensitized with an MAb to the serotype b-specific polysaccharide antigen agglutinated with whole cells of serotype b A. actinomycetemcomitans and P. gingivalis, but not with heated and trypsinized cells of P. gingivalis. The simple and rapid latex agglutination assay using MAbs may be useful for the identification of A. actinomycetemcomitans.
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Citations
Feb 14, 1992·Journal of Immunological Methods·S Yazawa, H Ohkawara
Jan 1, 1999·Oral Microbiology and Immunology·N YamaguchiT Koga
Jun 1, 1995·Oral Microbiology and Immunology·Y NakanoT Koga
Mar 1, 2003·Journal of Periodontal Research·Yuko OhguchiToshihide Noguchi
Jan 10, 1998·Infection and Immunity·Y YoshidaT Koga
Dec 1, 1995·Infection and Immunity·N YamaguchiT Koga
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