PMID: 7524130Jan 1, 1994Paper

Monoclonal antibody M3 used in tissue polypeptide-specific antigen assay for the quantification of tissue polypeptide antigen recognizes keratin 18

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
J M BonfrerD Ivanyi

Abstract

Recently, a new 'specific tissue polypeptide antigen (TPA)' test was introduced and designated tissue polypeptide-specific antigen (TPS); it is based on the monoclonal antibody (MAb) anti-TPS, M3. We have tested the specificity of this antibody by immunocyto- and immunohistochemistry, gel electrophoresis and immunoblotting. MAb M3 bound to intermediate filaments of epithelial cells and revealed a staining pattern identical to cytokeratin (CK) 18-specific MAb (DE-K18) on tissue sections of various human tissues. On immunoblots of proteins extracted from various epithelial cell lines, M3 reacted with a 45-kD protein corresponding to CK18, and on immunoblots of proteins isolated from MCF-7 culture fluid M3 stained three bands, 45, 33 and 29 kD. The same bands were stained with CK18-specific MAb, indicating that they represent CK18 and its degradation products. TPA, used as a tumor marker in clinical diagnoses and follow-up, was shown to be a degradation product of CK 8, 18 and 19. In contrast to TPA, MAb M3 did not stain CK8 and CK19 present on immunoblots.

Citations

Jun 30, 1998·International Journal of Cancer. Journal International Du Cancer·C TempferC Kainz
Dec 1, 1996·Cancer Metastasis Reviews·R G OshimaC Caulín
May 1, 1996·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·T TedoneS J Reshkin
Sep 15, 2004·Alcoholism, Clinical and Experimental Research·A Gonzalez-QuintelaJ Forteza
Nov 16, 2006·Liver International : Official Journal of the International Association for the Study of the Liver·Arturo Gonzalez-QuintelaEsteban Otero
Oct 19, 1999·Thyroid : Official Journal of the American Thyroid Association·S SuzukiA Tsuchiya
Sep 1, 2000·Alcoholism, Clinical and Experimental Research·A González-QuintelaS Lojo
Mar 17, 2007·Critical Reviews in Clinical Laboratory Sciences·Angeliki SarandakouDemetrios Rizos
Mar 28, 2012·Current Opinion in Gastroenterology·Pavel StrnadNam-On Ku
Jan 1, 1995·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·A van Dalen
Jul 21, 2012·International Journal of Cancer. Journal International Du Cancer·Soo Kyung AhnDong-Young Noh
Sep 4, 1998·Cancer Detection and Prevention·B NismanJ Lafair
Sep 1, 1997·Medical and Pediatric Oncology·W RebhandlE Horcher
Jun 21, 2011·Alcoholism, Clinical and Experimental Research·Arturo Gonzalez-QuintelaFrancisco Gude
Mar 2, 2006·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·T UenoS Linder
Jan 22, 2008·Oral Oncology·Sharada S SawantMilind M Vaidya

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