Monoclonal antibody mapping of the envelope glycoprotein of the dengue 2 virus, Jamaica

Virology
J T RoehrigR G Kelly

Abstract

Although dengue (DEN) virus is the etiologic agent of dengue fever, the most prevalent vector-borne viral disease in the world, precise information on the antigenic structure of the dengue virion is limited. We have prepared a set of murine monoclonal antibodies (MAbs) specific for the envelope (E) glycoprotein of DEN 2 virus and used these antibodies in a comprehensive biological and biochemical analysis to identify 16 epitopes. Following domain nomenclature developed for the related flavivirus, tick-borne encephalitis, three functional domains were identified. Five epitopes associated with domain A were arranged in three spatially independent regions. These A-domain epitopes were destroyed by reduction, and antibodies reactive with these epitopes were able to block virus hemagglutination, neutralize virus infectivity, and block virus-mediated cell membrane fusion. Domain-A epitopes were present on the full-length E glycoprotein, a 45-kDa tryptic peptide representing its first 400 amino acids (aa) and a 22-kDa tryptic peptide representing at least aa 1-120. Four epitopes mapped into domain B, as determined by their partial resistance to reduction and the localization of these epitopes on a 9-kDa tryptic or chymotryptic peptide...Continue Reading

References

Jun 1, 1989·The American Journal of Tropical Medicine and Hygiene·B L InnisC Hemachudha
Jan 1, 1985·The American Journal of Tropical Medicine and Hygiene·E A HenchalW E Brandt
Sep 24, 1987·Journal of Immunological Methods·A H RossH Koprowski
Mar 1, 1987·The American Journal of Tropical Medicine and Hygiene·B M KaufmanK H Eckels
Jan 1, 1974·The Journal of General Virology·J F ObijeskiF A Murphy
May 1, 1982·The American Journal of Tropical Medicine and Hygiene·M K GentryJ M Dalrymple

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Citations

May 13, 2008·Expert Reviews in Molecular Medicine·Theodore C Pierson, Michael S Diamond
Dec 11, 2007·Nature Biotechnology·Wayne A Marasco, Jianhua Sui
Apr 27, 2005·Nature Medicine·Theodore OliphantMichael S Diamond
Mar 24, 2006·Viral Immunology·Joseph E BlaneyStephen S Whitehead
Jun 9, 2004·Journal of Clinical Microbiology·David W C BeasleyAlan D T Barrett
Apr 7, 2006·Journal of Clinical Microbiology·Li-Jung ChienGwong-Jen J Chang
Sep 16, 2006·Journal of Virology·Karin StiasnyFranz X Heinz
Jun 7, 2013·Journal of Virology·Zhiqing WangMichael G Rossmann
Jun 4, 2010·Journal of Virology·Jiraphan JunjhonNopporn Sittisombut
Jul 2, 2010·Journal of Virology·Soila Sukupolvi-PettyMichael S Diamond
Oct 29, 2010·Journal of Virology·Claire M MidgleyGavin R Screaton
Nov 27, 2004·Journal of Virology·Wayne D Crill, Gwong-Jen J Chang
Feb 14, 2004·Journal of Virology·Poonsook KeelapangNopporn Sittisombut
Dec 23, 2004·Journal of Virology·Yorgo ModisStephen C Harrison
Jun 30, 2011·PLoS Neglected Tropical Diseases·Ruklanthi de AlwisAravinda M de Silva
Apr 25, 2012·PLoS Neglected Tropical Diseases·Luana de BorbaClaudia N Duarte Dos Santos
May 9, 2012·PLoS Neglected Tropical Diseases·Pi-Chun LiHan-Chung Wu

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