PMID: 7529035Aug 1, 1994Paper

Monoclonal antibody-mediated inhibition of RNA binding and annealing activities of HIV type 1 nucleocapsid protein

AIDS Research and Human Retroviruses
V TanchouR Benarous

Abstract

Retroviral nucleocapsid (NC) proteins are highly basic, with one or two zinc fingers, and are required for virion formation, genomic RNA dimerization and packaging, and replication primer tRNA annealing to the viral RNA. We report here the first characterization of monoclonal antibodies directed against a retroviral nucleocapsid protein and their use to study the structure-function relationships of the nucleocapsid protein NCp7 of HIV-1. Four anti-NCp7 monoclonal antibodies (MAbs) have been generated by using NCp7 of HIV-1. The epitope targets of these MAbs were mapped using ELISA and BIAcore techniques. Whereas three of them are specific for epitopes located in the N and C termini of NCp7, the fourth one appears to be conformation specific. Interestingly, only two of these MAbs, the conformation-specific one and the MAb recognizing an N-terminal epitope are able to inhibit the RNA-binding and annealing activities of NCp7 as well as strong-stop DNA synthesis in vitro. The binding of the two other MAbs onto NCp7 either has no effect or enhances the NCp7-RNA interactions. These MAbs also display a differential recognition of the Gag polyprotein precursor, which makes them useful tools for studying NC protein maturation in the cou...Continue Reading

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Citations

Mar 22, 2003·Trends in Microbiology·Rebecca L Rich, David G Myszka
Jun 10, 2000·European Journal of Biochemistry·H de RocquignyB P Roques
Aug 25, 2000·AIDS Research and Human Retroviruses·H De RocquignyB P Roques
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·S DruillennecM C Fournié-Zaluski
Jan 10, 1998·The Journal of Biological Chemistry·H de RocquignyB P Roques
Mar 29, 2014·European Journal of Microbiology & Immunology·N V SrideviV A Srinivasan
Apr 10, 1999·The Journal of Biological Chemistry·S DruillennecB P Roques
Jul 19, 2001·Physiological Genomics·M DasJ Pelletier

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