Monoclonal Antibody Production Against Vimentin by Whole Cell Immunization in a Mouse Model

Iranian Journal of Biotechnology
Marzieh Rezaei, Abbas Ghaderi

Abstract

Pancreatic carcinoma is the fourth-leading cause of cancer death in the United States and due to its late presentation, only few patients would be candidates for the curative treatment of pancreactomy. Monoclonal antibodies have brought hope to targeted therapy. To identify new biomarkers, a panel of monoclonal antibodies was generated against newly established cell line, Faraz-ICR from a patient with pancreatic acinar cell carcinoma. Balb/c female mice were immunized with Faraz-ICR cell line and their spleenocytes fused with SP2/0 myeloma cell line. Highly reactive hybridoma producing antibodies against Faraz-ICR was detected using ELISA, immunofluorescence staining and flow cytometry. Western blot and 2D immunoblot were utilized for further characterization of the target antibodies. Among highly reactive clones, the reactivity of 7C11 clone was assessed in comparison to other epithelial tumors. The antibody isotype was IgM that reacted with a 55 kDa protein in western blot analysis. To further characterize the target antigen, immunoproteome of the Faraz-ICR cell line was performed. By LC-MS analysis, the target of 7C11 clone was identified to be vimentin. Pancreatic cancer is a highly lethal malignancy with no reliable biomar...Continue Reading

References

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Methods Mentioned

BETA
Enzyme-linked Immunosorbent Assay
ELISA
protein assay
X-ray
flow cytometry

Software Mentioned

FlowJo
Mascot

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