PMID: 8440524Feb 1, 1993Paper

Monoclonal antibody prophylaxis against the in vivo toxicity of ricin in mice

Immunological Investigations
T C ChanhJ F Hewetson

Abstract

A BALB/c murine IgG1 monoclonal antibody, designated BG11-G2, specific for ricin was generated. BG11-G2 antibody did not bind to either purified ricin chain A or chain B, but recognized an antigenic determinant whose conformation requires the combination of the two chains in the formation of the native ricin molecule. It did not react with the protein synthesis inhibitor, T-2 mycotoxin, or with the sodium channel blockers, saxitoxin and tetrodotoxin. As little as 0.78 micrograms/ml of BG11-G2 IgG1 anti-ricin monoclonal antibody completely protected against the in vitro toxicity of ricin as determined by [3H]leucine uptake in EL-4 cell assays. Passive intraperitoneal infusion of purified BG11-G2 antibody into BALB/c mice one day prior to a lethal challenge with ricin considerably delayed the onset of toxicity and death. Better protection was obtained with BG11-G2 infused before and after ricin challenge.

References

Apr 1, 1991·Toxicology and Applied Pharmacology·T C ChanhJ F Hewetson
Jun 1, 1989·The Journal of Clinical Investigation·R I HuotT C Chanh
Jan 1, 1989·Journal of Toxicology and Environmental Health·R I HuotT C Chanh
Sep 1, 1989·Toxicology and Applied Pharmacology·T C ChanhJ F Hewetson
Aug 6, 1987·Nature·S Olsnes
May 9, 1972·Biochimica Et Biophysica Acta·G L Nicolson, J Blaustein
Jan 1, 1984·Journal of Cellular Biochemistry·R L BarchiR E Furman
Jan 1, 1982·Immunological Reviews·E S VitettaJ W Uhr

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Citations

Nov 1, 1993·Immunopharmacology·T C Chanh, J F Hewetson
May 23, 2006·Infection and Immunity·Carolyn R McGuinness, Nicholas J Mantis
Mar 14, 2013·BioMed Research International·Wei-Gang HuJohn W Cherwonogrodzky
Jun 7, 2005·Advanced Drug Delivery Reviews·Nicholas J Mantis
Feb 1, 1995·Human & Experimental Toxicology·G D GriffithsD G Upshall
Apr 2, 2005·Nature Reviews. Drug Discovery·James C BurnettSina Bavari
Jul 16, 2013·The Journal of Biological Chemistry·Yuwei ZhuMaikun Teng
Jul 24, 2012·Toxins·Siu-Kei Chow, Arturo Casadevall

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