PMID: 3750342Jan 1, 1986Paper

Monoclonal antibody specific for cyanoginosin-LA: preparation and characterization

Toxicon : Official Journal of the International Society on Toxinology
R KfirD P Botes

Abstract

The toxin cyanoginosin-LA (MW 909), isolated from Microcystis aeruginosa, was successfully conjugated with polylysine and muramyl dipeptide to form a high molecular weight complex consisting of a hapten, a carrier and a built-in adjuvant. This complex was used for the immunization of mice. Monoclonal antibodies specific for cyanoginosin-LA were produced using the hybridoma technique. The ten most efficient producers of these antibodies were further characterized and the monoclonal antibody produced by them was found to be identical on the basis of additivity test, isoelectric focussing and sub-class immunoglobulin typing results. The anti-cyanoginosin-LA monoclonal antibody focussed at a pH of approximately 6.55 and was found to belong to the mouse immunoglobulin subclass IgM. Large scale production of the antibody (in vivo) was followed by purification with ammonium sulphate precipitation and high performance liquid chromatography. The anticyanoginosin-LA monoclonal antibody, when reacted with six variants of cyanoginosin (other than cyanoginosin-LA), bound all with equal efficiency.

References

Jan 1, 1985·Toxicon : Official Journal of the International Society on Toxinology·R KfirG Osthoff
Jun 24, 1983·Science·D N SlatkinH W Siegelman
Nov 1, 1981·Naunyn-Schmiedeberg's Archives of Pharmacology·M T RunnegarJ Silver
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Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R ArnonL Chedid

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Citations

Nov 1, 1988·Toxicology and Applied Pharmacology·W H AdamsH W Siegelman
Jan 1, 1988·Toxicon : Official Journal of the International Society on Toxinology·W W CarmichaelR E Moore
Jan 1, 1989·Toxicon : Official Journal of the International Society on Toxinology·R D StonerH W Siegelman
May 1, 1994·Toxicon : Official Journal of the International Society on Toxinology·J R Lin, F S Chu
Jan 4, 2001·Toxicon : Official Journal of the International Society on Toxinology·P MehtoJ Meriluoto
Nov 14, 1997·Bioorganic & Medicinal Chemistry·J E SheppeckA R Chamberlin
Oct 1, 1990·Letters in Applied Microbiology·L A LawtonG A Codd
Jun 19, 2014·Environmental Science & Technology·Ingunn A SamdalChristopher O Miles
Jul 15, 2020·Journal of Biochemical and Molecular Toxicology·Piyush KumarSanjay Kumar
Dec 15, 2020·Journal of Microbiological Methods·Caitlin S RomanisBrett A Neilan
Aug 1, 1989·Applied and Environmental Microbiology·F S ChuW W Carmichael

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