Monoclonal antibody to galactosylceramide: discrimination of structural difference in the ceramide moiety.

FEBS Letters
H NakakumaK Takatsuki

Abstract

A mouse monoclonal antibody (mAb) was developed against monohexaosylceramide. This mAb differentially reacted on thin-layer chromatograms with 3 types of galactosylceramide (GalCer) obtained from bovine brain. Structural analysis of the 3 glycolipids revealed that they consisted of the same galactose and sphingosine but of apparently different fatty acids. Among the GalCers, the mAb reacted with teh two GalCers which contained alpha-hydroxy fatty acids, but not with GalCer composed of nonhydroxy fatty acids. These findings suggest that not only that the mAb discriminated the fatty acid composition in the ceramide moiety of GalCer, but also that the ceramide structure defines the immunological epitope as it is known to do for the carbohydrate moiety of glycosphingolipid.

References

Feb 1, 1988·Japanese Journal of Cancer Research : Gann·R YoshimuraM Akagi
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R KannagiS Hakomori
Jan 1, 1981·Annual Review of Biochemistry·S Hakomori
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·B RanschtW Seifert

❮ Previous
Next ❯

Citations

Aug 1, 1996·Glycoconjugate Journal·C A Lingwood
Feb 1, 1991·Glycoconjugate Journal·A KalisiakD A Scheinberg
Apr 1, 1992·Molecular and Biochemical Parasitology·E Barreto-BergterH Egge
Apr 19, 2002·Journal of Neuroimmunology·Nathalie Escande-BeillardJosé Boucraut
Jan 5, 2002·Molecular and Biochemical Parasitology·Luz María López-MarínMamadou Daffé
May 20, 1996·AIDS Research and Human Retroviruses·N SeddikiL Gattegno
Nov 1, 2001·Clinical and Diagnostic Laboratory Immunology·R SaavedraL M López-Marín
Nov 29, 2002·Eukaryotic Cell·Eric MaréchalJacques Joyard
Jan 1, 1991·International Reviews of Immunology·M H Ravindranath, D L Morton
Feb 25, 2005·FEMS Microbiology Letters·Maria Helena Villas-BoasEliana Barreto-Bergter
Jun 30, 2006·Veterinary Parasitology·Akiko UemuraHiroshi Kodama
Aug 18, 2020·Biochimie·Motohide MurateToshihide Kobayashi
Dec 23, 2009·Biochimica Et Biophysica Acta·Hiroko Hama

❮ Previous
Next ❯

Related Concepts

Related Feeds