PMID: 6538613Jan 1, 1984Paper

Monoclonal antibody to the hen oviduct progesterone receptor produced following in vitro immunization

Journal of Steroid Biochemistry
P D DickerB W O'Malley

Abstract

The progesterone receptor of the hen oviduct is composed of two non-identical hormone-binding polypeptide subunits, A (Mr = 79,000) and B (Mr = 108,000). We used a highly purified preparation of B to immunize mouse spleen cells in vitro. After 5 days in culture, the cells were fused with SP2/0-Ag 14 myeloma cells. The resultant hybridomas were screened using an enzyme linked immunosorbent solid phase assay, and those hybridomas producing antibodies binding to the immunogen were cloned by limiting dilution. One such clone, 9B3-12, secreted an antibody of immunoglobulin class IgM, which binds to B. This was indicated by the ability of the antibody to increase the rate of sedimentation coefficient of the B subunit. Further, when the proteins in the B preparation were separated by electrophoresis and blotted onto nitrocellulose filters the antibody bound to a protein of 108,000 daltons. The antibody produced by 9B3-12 also reacted with subunit A and with the human progesterone receptor but failed to bind to the chick liver glucocorticoid receptor or to progesterone in the absence of its receptor.

References

May 1, 1979·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·I KeydarH J Brenner
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jul 1, 1976·European Journal of Immunology·G Köhler, C Milstein
Jan 1, 1966·Experimental Cell Research·J W Littlefield
Sep 16, 1981·Biochemical and Biophysical Research Communications·N L WeigelB W O'Malley
Sep 30, 1982·Journal of Immunological Methods·C L Reading
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G L GreeneE V Jensen
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·F LogeatE Milgrom
Nov 1, 1982·Endocrinology·P GrandicsG Litwack
Jan 1, 1996·Breast Cancer Research and Treatment·M A FanelliD R Ciocca

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