PMID: 6194814Aug 30, 1983

Monoclonal antibody to the region of fibronectin involved in cross-linking to human fibrin

Biochemistry
J H SobelR E Canfield

Abstract

A cross-link-containing fragment (alpha XLCNBr), derived from the alpha-polymer component of human fibrin following CNBr digestion, has been isolated and characterized. NH2-terminal sequence studies of three alpha XLCNBr derivatives, each prepared by a different method, indicate that the A alpha-chain regions comprised of residues 241-476 (CNBr VIII) and 518-584 (CNBr X) are the major constituents of the cross-linked fragments examined. Evidence for at least two additional sequences suggests that A alpha 208-235 (CNBr V) and A alpha 585-610 (CNBr XI) may have auxiliary roles in alpha-polymer formation. When alpha XLCNBr was used as an immunogen for the production of murine hybridoma cell lines, two groups of antibodies were obtained. The majority of supernatants from primary hybridoma cultures did not discriminate between fibrinogen and alpha XLCNBr and appeared to contain antibodies directed against determinants within either CNBr VIII or CNBr X [see Ehrlich, P. H., Sobel, J. H., Moustafa, Z. A., & Canfield, R. E. (1983) Biochemistry (following paper in this issue)]. Several supernatants from primary hybridoma cultures, however, did exhibit significant binding toward the alpha-polymer derivative in the absence of demonstrable ...Continue Reading

References

May 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M PierresR N Germain
Jan 1, 1979·Thrombosis Research·P J Gaffney, A N Whitaker
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Nov 1, 1979·The Journal of Clinical Investigation·H L NosselR E Canfield
Oct 12, 1978·Nature·B BlombäckL Therkildsen
Dec 1, 1978·Hoppe-Seyler's Zeitschrift für physiologische Chemie·A H Henschen
Apr 29, 1975·Biochimica Et Biophysica Acta·M W MosessonR M Huseby
Jun 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·R Chen, R F Doolittle
Apr 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J J PisanoY Nagai
Oct 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·H L NosselV P Butler
Sep 15, 1980·Thrombosis Research·D H CorcoranP A McKee
Sep 15, 1981·Analytical Biochemistry·J A Koehn, R E Canfield

Citations

Dec 4, 2003·Thrombosis Research·Chun-Che ShihShing-Jong Lin
Jan 1, 1986·Critical Reviews in Oncology/hematology·A Z Budzynski
Jul 13, 2005·Journal of Biomedical Materials Research. Part a·Chun-Che ShihShing-Jong Lin
Aug 9, 1996·The Journal of Biological Chemistry·J H Sobel, M A Gawinowicz
Jan 8, 1985·The Journal of Comparative Neurology·L R Williams, S Varon

Related Concepts

Monoclonal Antibodies
Immune complex
Antigenic Specificity
Cyanogen Bromide
Enzyme-Linked Immunosorbent Assay
Antithrombin I
Fibronectins
Guanidines
Peptide Fragments
Guanidine Sulfite (1: 1)

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