Monoclonal immunoglobulin A antibody to the major outer membrane protein of the Chlamydia trachomatis mouse pneumonitis biovar protects mice against a chlamydial genital challenge

Vaccine
S PalLuis M de la Maza

Abstract

In order to analyze the protective role that IgA may play in a chlamydial infection two IgA monoclonal antibodies (mAb), MoPn 4-2 and MoPn 13-2, were raised against the major outer membrane protein (MOMP) of the Chlamydia trachomatis mouse pneumonitis (MoPn) biovar. mAb MoPn 4-2 was found to be serovar specific while mAb MoPn 13-2 was species specific. mAb MoPn 4-2 recognized a surface exposed conformational epitope as shown by its ability to bind to native EBs and nonreduced MOMP while failing to bind to heat and trypsin treated EBs, to reduced MOMP and to synthetic MOMP peptides. In contrast, mAb MoPn 13-2 recognized a nonconformational epitope since it was able to bind treated EBs, to reduced MOMP and to the synthetic peptide MTTWNPTISGSGI located in variable domain 4 of the MOMP. Both mAbs agglutinated intact EBs and had in vitro neutralizing activity. However, mAb MoPn 4-2 had a 20-fold higher in vitro neutralizing ability when compared to mAb MoPn 13-2 (50% neutralization at 5 micrograms ml-1 vs 100 micrograms ml-1). In an in vitro in vivo infectivity assay, mAb MoPn 4-2 protected mice against infertility when C. trachomatis MoPn elementary bodies were preincubated with the mAb before inoculation. In addition, passive tra...Continue Reading

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Citations

Sep 21, 2000·Journal of Reproductive Immunology·K W Beagley, P Timms
May 16, 1998·Trends in Microbiology·A Casadevall
Jun 19, 2002·Protein Science : a Publication of the Protein Society·María José Rodríguez-MarañónLuis M de la Maza
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Nov 8, 2014·Journal of Peptide Science : an Official Publication of the European Peptide Society·Shanli ZhuLifang Zhang
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Nov 22, 2016·American Journal of Reproductive Immunology : AJRI·Charles W ArmitageKenneth W Beagley

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