Monoclonal murine anti-nucleic acid antibody with double-stranded specificity

Molecular Immunology
D W Ballard, E W Voss

Abstract

The NZB/NZW F1 murine model for the autoimmune disease systemic lupus erythematosus (SLE) has been employed in somatic cell hybridizations to develop hybridoma autoantibodies with double-stranded (ds) DNA specificity. Monoclonal anti-DNA antibodies from one hybridoma cell line were purified and analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and isoelectric focusing. Results of comparative binding studies with tritiated [3H]-colicin E1 plasmid DNA probes suggested preferential binding for the native DNA conformation relative to single-stranded DNA. [3H]dsDNA binding was inhibited by several ribohomopolymers (poly G, U and I) but not by free nucleotides, indicating that the phosphodiester-ribose backbone may contribute to the binding specificity of the clonotype.

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Citations

Mar 10, 1989·Journal of Immunological Methods·Y N Vaishnav, A Antony
Feb 1, 1985·The Journal of Clinical Investigation·R S Schwartz, B D Stollar
Jan 1, 1986·Annals of the New York Academy of Sciences·B D Stollar, R S Schwartz
Jan 1, 1989·International Reviews of Immunology·B D Stollar
Jul 12, 2014·Expert Review of Molecular Diagnostics·Zonglin HuChristopher A Elkins
Jan 1, 1986·CRC Critical Reviews in Biochemistry·B D Stollar
May 1, 1983·Human Pathology·D KofflerI Faiferman

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