Monoclonal paraprotein influences baseline B-cell repertoire diversity and perturbates influenza vaccination-induced B-cell response

Experimental Hematology
Sarah M TeteNicolaas A Bos

Abstract

Monoclonal gammopathy of undetermined significance (MGUS) arises from a clonal expansion of plasma cells in the bone marrow, secreting monoclonal (M) paraprotein. It is associated with increased susceptibility to infections, which may reflect altered B-cell repertoire. To investigate this, we examined the immunoglobulin (Ig) M, IgG, and IgA B-cell repertoire diversity in MGUS at baseline and after influenza vaccination (n = 16) in comparison with healthy controls (HCs; n = 16). The Complementary Determining Region 3 region of the immunoglobulin heavy chain variable region gene was amplified and B-cell spectratypes analyzed by high-resolution electrophoresis. Spectratype Gaussian distribution, kurtosis, and skewness were quantified to measure repertoire shifts. Both HC and MGUS baseline spectratypes show interindividual variability that is more pronounced in the IGHG and IGHA repertoires. Overall, baseline B-cell repertoire is more altered in MGUS, with oligoclonality observed in 50% (p = 0.01). Postvaccination, significant differences emerged in MGUS in relation to M-protein levels. High M-protein concentration is associated with a more oligoclonal IgG and IgA response at day 7 postvaccination, and, in contrast to HCs, vaccinat...Continue Reading

References

Mar 22, 2002·Nature Reviews. Immunology·A BendelacJ F Kearney
Aug 16, 2003·Science·Hedda WardemannMichel C Nussenzweig
Nov 26, 2003·The Journal of Experimental Medicine·Madhav V DhodapkarMatthew D Geller
Aug 20, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Shaji KumarThomas E Witzig
Feb 8, 2006·Annals of the New York Academy of Sciences·Madhav V Dhodapkar
Mar 31, 2006·The New England Journal of Medicine·Robert A KyleL Joseph Melton
Mar 29, 2007·The Journal of Experimental Medicine·Radek SpisekMadhav V Dhodapkar
Jun 22, 2007·The New England Journal of Medicine·Robert A KyleS Vincent Rajkumar
Nov 7, 2008·Aging Cell·Kate L GibsonDeborah K Dunn-Walters
Feb 16, 2010·Cytometry. Part B, Clinical Cytometry·Bruno PaivaAlberto Orfao
Apr 15, 2010·The Journal of Clinical Investigation·Davide CortiAntonio Lanzavecchia
Jan 12, 2011·The Journal of Experimental Medicine·Jens WrammertPatrick C Wilson

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Citations

Oct 13, 2020·Hematology Reports·Alessandra RomanoFrancesco Di Raimondo

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