Abstract
We used suncus (Suncus murinus; house musk shrew) to generate partner cells for cell fusion to produce suncus monoclonal antibodies. Suncus are insectivores that are genetically distant to rodents, and recognize antigens and epitopes that are not immunogenic in mice and rats, which are the animals most commonly used in basic life science research and from which monoclonal antibodies are usually produced. To date, monoclonal antibodies from suncus have not been generated due to the lack of a plasmacytoma fusion partner. To obtain suncus plasmacytoma cell lines suitable as a cell fusion partner, we injected suncus at both sides of the tail base with antigen emulsion, collected the lymph nodes and spleens, and cultured the cells to obtain immortalized lymphoid cell lines visually resembling mouse SP2/0-Ag14 myeloma cells. Three suncus immunized with the antigen provided 4 cell lines of suncus plasmacytoma, but they did not secrete immunoglobulins. Antibody-producing hybrid cells were generated from these cell lines using a cell fusion technique. Using one of the cell lines as a fusion partner, we obtained six lines of immunoglobulin-producing hybrid cells which secreted an unidentified monoclonal IgG. When these 6 lines were used ...Continue Reading
References
Nov 16, 1978·Nature·M ShulmanG Köhler
Aug 7, 1975·Nature·G Köhler, C Milstein
Aug 31, 1992·Neuroscience Letters·N KarasawaI Nagatsu
Oct 1, 1992·The Journal of Pathology·Y SadoT Okigaki
Jan 1, 1991·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·Y SadoT Okigaki
Jun 24, 1988·Science·T J Raybould, M Takahashi
Jan 1, 1986·Methods in Enzymology·L De ClercqH Bazin
Jan 1, 1983·Hybridoma·A M Lebacq-VerheydenH Bazin
Jun 1, 1982·The Journal of Cell Biology·J V KilmartinC Milstein
Sep 15, 1980·Experientia·K AndoT A Uchida
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·H Spieker-PoletK L Knight
Apr 1, 1995·Cell Structure and Function·Y KishiroY Sado
Feb 1, 1995·Idengaku zasshi·A IshikawaT Namikawa
Jul 1, 1993·Jikken dobutsu. Experimental animals·K GotoR Harasawa
Oct 1, 1995·Histochemistry and Cell Biology·Y SadoY Ninomiya
Jul 1, 1996·Virchows Archiv : an International Journal of Pathology·S RaufY Sado
Jun 1, 1997·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·M KagawaY Sado
Jun 1, 1997·The Journal of Pathology·Y SadoY Ninomiya
Jul 18, 2000·The Journal of Biological Chemistry·A BoutaudB G Hudson
Jul 12, 2001·The Journal of Heredity·A KuroiwaY Matsuda
Jun 18, 2004·Kidney International·Takayuki KohdaYoshikazu Sado
Mar 1, 2007·Acta Histochemica Et Cytochemica·Yoshikazu SadoHiroyuki Omori
Jun 27, 2015·PloS One·Chayon GoswamiIchiro Sakata
May 27, 2016·Temperature : Multidisciplinary Biomedical Journal·John A RuddPaul Lr Andrews