PMID: 15218043Jun 26, 2004Paper

Monocyte activation by Porphyromonas gingivalis LPS in aggressive periodontitis with the use of whole-blood cultures

Journal of Dental Research
R MahanondaS Pichyangkul

Abstract

In this study, we re-visited the issue of hyper-responsiveness of monocytes to bacterial lipopolysaccharide (LPS) in aggressive periodontitis patients. We used whole-blood cultures to compare monocyte activation by Porphyromonas gingivalis LPS between Thai subjects with generalized aggressive periodontitis and those without periodontitis. Upon stimulation with P. gingivalis LPS, expression of co-stimulatory molecules on monocytes and expression of CD69 on NK and gamma delta T-cells were analyzed by flow cytometry, and the production of interleukin-1 beta and prostaglandin E(2) was monitored by ELISA. LPS stimulation resulted in a dose-dependent up-regulation of CD40, CD80, and CD86 on monocytes, and up-regulation of CD69 on NK cells and gamma delta T-cells in both the periodontitis and non-periodontitis groups. The levels of activation markers and the mediator production after LPS stimulation were quite similar for both groups. In conclusion, we did not observe hyper-responsiveness of monocytes to P. gingivalis LPS challenge in Thai patients with aggressive periodontitis.

References

Oct 12, 1995·Journal of Immunological Methods·N Petrovsky, L C Harrison
Jan 1, 1997·Journal of Clinical Periodontology·K S KornmanG W Duff
Mar 1, 1997·Journal of Periodontology·G D SempowskiT M Blieden
Jul 1, 1998·International Reviews of Immunology·F FossiezS Lebecque
Sep 2, 1998·Annals of Periodontology·K S Kornman, F S di Giovine
Apr 8, 1999·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·S Offenbacher, G E Salvi
Aug 13, 1999·Journal of Periodontal Research·P M PreshawJ D Walters
Sep 1, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R L Van bezooijenC W Löwik
Oct 16, 1999·Periodontology 2000·P M Fives-TaylorC Brissette
Dec 10, 1999·Journal of Clinical Periodontology·G M GalbraithJ P Pandey
Jun 23, 2000·Annals of Periodontology·M S Tonetti, A Mombelli
Jan 11, 2001·Journal of Immunological Methods·S PichyangkulD S Walsh
Jan 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tim G A M WolfsCornelis van 't Veer
Jan 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yonca BulutMoshe Arditi
Nov 26, 2002·Journal of Clinical Periodontology·Hideaki TaiHiromasa Yoshie
Apr 4, 2003·Clinical Immunology : the Official Journal of the Clinical Immunology Society·A B J GroeneveldC E Hack
Oct 31, 2003·Journal of Periodontology·Jung-Wook LeeChong-Kwan Kim
Feb 21, 2004·Journal of Periodontology·Orasa AnusaksathienRawee Teanpaisan

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Citations

May 5, 2011·Microbial Pathogenesis·Nina ScheresVincent Everts
Jan 31, 2006·Journal of Clinical Periodontology·A GustafssonK Bergström
Feb 1, 2011·Journal of Investigative and Clinical Dentistry·Daniel GrenierGuy Gagnon
Apr 17, 2014·Journal of Clinical Periodontology·Wan-Chien ChengLeonie S Taams
Feb 22, 2012·European Journal of Microbiology & Immunology·O CohavyS R Targan
Oct 3, 2006·The Journal of the American Dental Association·Ryan T Demmer, Moïse Desvarieux
Aug 28, 2007·Journal of Dental Research·C S Patil, K L Kirkwood
Dec 30, 2020·International Journal of Molecular Sciences·Andreas SeidelHelga Schmetzer

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