Monocyte activation in patients with age-related macular degeneration: a biomarker of risk for choroidal neovascularization?

Archives of Ophthalmology
Scott W CousinsKarl G Csaky

Abstract

To evaluate the activation state of macrophage function in patients with age-related macular degeneration (AMD) by quantifying the production of the proinflammatory and angiogenic factor tumor necrosis factor alpha (TNF-alpha) and by correlating its expression with dry and wet AMD. Circulating monocytes were obtained from the blood of patients with AMD or age-matched control subjects by gradient centrifugation. The monocytes were then analyzed for either TNF-alpha release from cultured macrophages in response to retinal pigment epithelium-derived blebs and cytokines or TNF-alpha messenger RNA content by reverse transcriptase-polymerase chain reaction. In human monocytes obtained from controls and AMD patients, TNF-alpha was expressed by freshly isolated monocytes and produced by macrophages in culture after stimulation with retinal pigment epithelium-derived blebs. However, wide variability in TNF-alpha expression was observed among different patients. Patients with monocytes that expressed the greatest amount of TNF-alpha demonstrated higher prevalence of choroidal neovascularization. Both controls and AMD patients vary in the activation state (defined as TNF-alpha expression) of circulating monocytes. Partially active monocyt...Continue Reading

Citations

May 5, 2005·Proceedings of the National Academy of Sciences of the United States of America·Gregory S HagemanRando Allikmets
Jan 7, 2014·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Kunbei LaiJian Ge
Apr 11, 2008·PPAR Research·Marisol Del V Cano, Peter L Gehlbach
Sep 23, 2014·Eye·M Gemenetzi, A J Lotery
Apr 26, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kousuke NodaAli Hafezi-Moghadam
Dec 12, 2012·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Lihteh WuMichel E Farah
May 21, 2013·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Rogil Jose de Almeida TorresDalton Bertolim Precoma
Jan 1, 2012·Asia-Pacific Journal of Ophthalmology·Tsz Kin NgChi Pui Pang
Oct 6, 2006·The New England Journal of Medicine·Paulus T V M de Jong
Feb 8, 2016·Cellular and Molecular Life Sciences : CMLS·Anu KauppinenKai Kaarniranta
May 12, 2012·Molecular Aspects of Medicine·Priyatham S MettuScott W Cousins
Oct 18, 2011·Experimental and Molecular Pathology·Kei MorohoshiSanta J Ono
Feb 1, 2006·Acta Ophthalmologica Scandinavica·Peep V AlgvereStefan Seregard
Oct 1, 2011·Seminars in Ophthalmology·Ahad FazelatJohn J Weiter
Nov 5, 2016·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Zhaojiang DuLi Wang
Feb 28, 2006·Survey of Ophthalmology·Larry A DonosoGregory Hageman
Apr 24, 2007·International Ophthalmology Clinics·Fina C Barouch, Joan W Miller
Jul 24, 2014·Cell Biology International·Qing QuGuo-Huang Fan
Mar 20, 2018·Journal of Neuroinflammation·Sara TouhamiFlorian Sennlaub
Sep 19, 2018·EMBO Molecular Medicine·Isha Akhtar-SchäferThomas Langmann
Aug 10, 2019·Annals of the New York Academy of Sciences·Wolfram EichlerInes Liebscher
Jul 10, 2017·Journal of Molecular Neuroscience : MN·Alexandre ValléeJean-Noël Vallée
Feb 14, 2019·International Journal of Molecular Sciences·Thierry LéveillardFlorian Sennlaub
May 28, 2020·International Journal of Molecular Sciences·Undral BuyandelgerIkuo Tooyama
Feb 6, 2019·Vestnik oftalmologii·N A Ermakova
Feb 6, 2020·International Journal of Molecular Sciences·Alexandre ValléeJean-Noël Vallée
Oct 8, 2020·Médecine sciences : M/S·Christophe RoubeixFlorian Sennlaub

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