PMID: 11918087Mar 29, 2002Paper

Monocyte chemoattractant protein-1 (MCP-1), secreted by bone marrow endothelial cells, induces chemoattraction of 5T multiple myeloma cells

Clinical & Experimental Metastasis
Karin VanderkerkenBen Van Camp

Abstract

Homing of multiple myeloma (MM) cells to the bone marrow (BM) requires transendothelial migration. In the present work we tested whether monocyte chemoattractant protein-1 (MCP-1) and CCR2, the high affinity receptor for MCP-1, are involved in this process. Murine 5T2 and 5T33MM cell lines were selected as source of MM cells and STR4, 10 and 12 of BM endothelial cells (BMEC). RT-PCR demonstrated transcripts for MCP-1 in BMEC and ELISA the presence of MCP-1 protein in culture medium. RNase protection assay showed mRNA expression for CCR2, and FACS analysis the presence of CCR2 protein on the MM cells. EC conditioned medium induced chemoattraction of MM cells, a phenomenon inhibited by anti-MCP-1 antibodies. In conclusion, MM cells express CCR2 and are attracted by MCP-1 secreted by BMEC. We suggest that local MCP-1 production by BMEC is one of the mechanisms involved in homing of myeloma cells to the BM.

Citations

Dec 18, 2002·Annals of the New York Academy of Sciences·Eline MenuKarin Vanderkerken
Jun 2, 2007·Journal of Cellular Biochemistry·Matthew CraigRobert D Loberg
Oct 2, 2007·Clinical & Experimental Metastasis·Fayth L MilesCarlton R Cooper
Jul 3, 2004·Virchows Archiv : an International Journal of Pathology·Hendrik R De RaeveKarin Vanderkerken
Mar 5, 2009·Journal of Cellular Biochemistry·Stefan RozelKenneth J Pienta
Mar 17, 2010·Clinical and Experimental Medicine·Toni ValkovićNives Jonjić
Sep 19, 2002·International Journal of Cancer. Journal International Du Cancer·Els Van ValckenborghKaren Vanderkerken
Jan 26, 2012·Expert Review of Hematology·Mahmoud R GaballaPaul G Richardson
Dec 1, 2010·Journal of Neuro-oncology·Xinmei ZhuHideho Okada
Dec 13, 2006·Cancer Metastasis Reviews·Matt J Craig, Robert D Loberg
Feb 26, 2008·Expert Opinion on Biological Therapy·Karin JöhrerRichard Greil
Mar 18, 2010·Journal of the National Cancer Institute·Jian ZhangKenneth J Pienta
Sep 16, 2017·Current Opinion in Hematology·Vanessa BianconiMatteo Pirro
Jul 9, 2003·Immunological Reviews·Karin VanderkerkenBen Van Camp
Mar 26, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Karin JöhrerRichard Greil

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