PMID: 6985943Jan 1, 1980Paper

Monocyte contamination in Ficoll-Hypaque mononuclear cell concentrates

Journal of Immunological Methods
S Soman, L S Kaplow

Abstract

Monocyte contamination in mononuclear concentrates prepared by Ficoll-Hypaque (F-H) centrifugation was assessed by the following criteria: (a) morphology (Wright-Giemsa stain), (b) function (phagocytosis), and (c) cytochemical staining (non-specific esterase both manually and by flow cytophotometry). Studies on F-H preparations from blood samples obtained from 50 randomly selected patients irmined by functional and cytochemical staining. Monocyte counts determined by flow cytophotometric studies of cells stained in suspension for esterase were very similar to those based on phagocytosis and manual cytochemical staining technics. It is concluded that: (1) manual Wright-Giemsa staining alone is not adequate to accurately quantitate monocyte populations in F-H concentrates, and (2) cytochemical staining and flow cytophotometry can be used for rapid and accurate quantitation of monocyte contamination in F-H mononuclear concentrates.

References

Jan 1, 1977·Journal of Immunological Methods·S B TuckerR E Jordon
Jul 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L S Kaplow, E Lerner
Jan 1, 1976·The Journal of Clinical Investigation·L T WilliamsR J Lefkowitz
Jan 1, 1976·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L S KaplowE Lerner
Dec 1, 1970·The Journal of Experimental Medicine·N AbramsonR S Cotran
Jan 1, 1968·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·R O MulhausenK Mellemgaard

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Citations

Jan 1, 1981·Journal of Immunological Methods·G GaudernackR Messner
May 1, 1983·Veterinary Immunology and Immunopathology·W J Bruyninckx, A M Blancquaert
Dec 1, 1984·Scandinavian Journal of Clinical and Laboratory Investigation·J Ikomi-KummI Wadsö

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