Monocyte-expressed urokinase regulates human vascular smooth muscle cell migration in a coculture model

Biological Chemistry
Angelika KuschI Dumler

Abstract

Interactions of vascular smooth muscle cells (VSMC) with monocytes recruited to the arterial wall at a site of injury, with resultant modulation of VSMC growth and migration, are central to the development of vascular intimal thickening. Urokinase-type plasminogen activator (uPA) expressed by monocytes is a potent chemotactic factor for VSMC and might serve for the acceleration of vascular remodeling. In this report, we demonstrate that coculture of human VSMC with freshly isolated peripheral blood-derived human monocytes results in significant VSMC migration that increases during the coculture period. Accordingly, VSMC adhesion was inhibited with similar kinetics. VSMC proliferation, however, was not affected and remained at the same basal level during the whole period of coculture. The increase of VSMC migration in coculture was equivalent to the uPA-induced migration of monocultured VSMC and was blocked by addition into coculture of soluble uPAR (suPAR). Analysis of uPA and uPAR expression in cocultured cells demonstrated that monocytes are a major source of uPA, whose expression increases in coculture five-fold, whereas VSMC display an increased expression of cell surface-associated uPAR. These findings indicate that upregu...Continue Reading

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Citations

Jul 11, 2006·Biotechnology Advances·Pradip K RoychoudhuryAshok Kumar
Oct 18, 2011·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·D M ScharnJ A van der Vliet
Jun 6, 2007·Biochemical and Biophysical Research Communications·Margret PateckiAngelika Kusch
Dec 6, 2011·Atherosclerosis·Bianca Fuhrman
Mar 28, 2006·Journal of Cellular and Molecular Medicine·M HeilW Schaper
Apr 22, 2011·Molecular and Cellular Biochemistry·Ming-Ying LuoWolfgang Schaper
Oct 27, 2020·Aging Medicine·Tianqi Ma, Yong-Ping Bai
Feb 19, 2021·Journal of Stroke·Dmitri NikitinDong-Eog Kim

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