Monocyte HLA-DR expression and neutrophil CD64 expression as biomarkers of infection in critically ill neonates and infants

Pediatric Research
Justin E JuskewitchW Charles Huskins

Abstract

Reduced monocyte HLA-DR expression and increased neutrophil CD64 expression have been proposed as biomarkers of infection. From 2009-2011, blood samples from neonatal intensive care unit (NICU) and pediatric intensive care unit (ICU) patients <1 y of age were collected at enrollment and during subsequent evaluation for suspected infection, if it occurred. Samples were analyzed for monocyte HLA-DR and neutrophil CD64 expression levels by flow cytometry. Forty-seven infants had study samples collected at enrollment; 26 infants had study samples collected at the time of a suspected infection. At enrollment, there was an inverse relationship between neutrophil CD64 expression and age (P ≤ 0.047). At the time of suspected infection, infants with an infection demonstrated a lower percentage of HLA-DR+ monocytes (P = 0.02, area under the curve (AUC) 0.78), higher percentage of CD64+ neutrophils (P = 0.009, AUC 0.81), and higher neutrophil CD64 expression levels (P = 0.04, AUC 0.75). Monocyte HLA-DR and neutrophil CD64 expression in critically ill infants are related to age and infection.

References

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Citations

Feb 28, 2018·Journal of Leukocyte Biology·Alberto García-SalidoManuel Ramírez-Orellana
Oct 13, 2017·Advances in Therapy·Jordi RelloSilvia Moyano
Dec 4, 2019·Expert Review of Clinical Pharmacology·Susanna Esposito, Nicola Principi
Apr 3, 2020·Surgical Infections·Nadine BeckmannCharles C Caldwell
Sep 21, 2019·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Stephania Vázquez RodríguezArturo Cérbulo-Vázquez
Aug 19, 2020·Biomarkers in Medicine·Mariza Z OikonomakouKarolina Akinosoglou
Jun 30, 2018·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Jan HalekJarmila Juranova
Dec 18, 2018·Frontiers in Pediatrics·Julie E HibbertTobias Strunk
Aug 4, 2018·Frontiers in Pediatrics·Simone S SchüllerOfer Levy

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Methods Mentioned

BETA
blood draw
flow cytometry
FACS

Software Mentioned

REDCap
REDCap ( Electronic Data Capture
FACSDiva

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