PMID: 7540642Jun 1, 1995Paper

Monocytes cultured in cytokine-defined environments differ from freshly isolated monocytes in their responses to IL-4 and IL-10

Journal of Leukocyte Biology
P H HartJ J Finlay-Jones

Abstract

To investigate the regulatory effects of the prototypic Th2 lymphocyte products and potential immunotherapeutic agents interleukin-4 (IL-4) and IL-10 on macrophages differentiated in vitro under different cytokine-defined environments, blood monocytes were incubated for 7 days in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF), macrophage colony-stimulating factor or IL-4. The effect of monocyte culture in the presence or absence of serum was also investigated. Functional responses by 7-day-cultured cells to IL-4, quantified as decreased CD14 expression and suppression of lipopolysaccharide (LPS)-induced tumor necrosis factor-alpha (TNF-alpha) and IL-1 beta production, and as a positive response, increased CD23 expression, were compared directly with the responses by monocytes from which they were derived. In response to IL-10, decreases in LPS-induced TNF-alpha and IL-1 beta production and reduction in the expression of major histocompatibility complex (MHC) class II antigens were examined. Seven-day cultured monocytes/macrophages showed (1) diminished TNF-alpha production in response to IL-10 but not IL-4 (2), diminished IL-1 beta production in response to both IL-4 and IL-10, and compared with fresh...Continue Reading

Citations

Feb 1, 1997·European Journal of Immunology·F ChapuisJ C Gluckman
Jan 7, 1998·Arthritis and Rheumatism·P Miossec, W van den Berg
Jul 19, 2000·Infection and Immunity·D F MorrisonM P Murtaugh
Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·H SasakiP Stashenko
May 18, 1999·International Immunology·M Adib-ConquyJ M Cavaillon
Nov 16, 2006·Cancer Biotherapy & Radiopharmaceuticals·Anna Maria BerghellaDomenico Adorno
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May 23, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Philippe MathurinHidekazu Tsukamoto
Jul 3, 2015·Clinical and Vaccine Immunology : CVI·Sameh Sarray, Wassim Y Almawi
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Oct 16, 2004·Expert Review of Anti-infective Therapy·Robert SnelgroveTracy Hussell
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Jan 10, 2002·Brain, Behavior, and Immunity·M J SchwarzM Ackenheil
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