Monocyte:T-cell interaction regulates human T-cell activation through a CD28/CD46 crosstalk

Immunology and Cell Biology
Lauren CharronAnne L Astier

Abstract

T-cell activation requires engagement of the T-cell receptor and of at least one costimulatory molecule. The key role of CD28 in inducing T-cell activation was reported several decades ago and the molecular mechanisms involved have now been well described. The complement regulator CD46 also acts as a costimulatory molecule for T cells but, in contrast to CD28, has the ability to drive T-cell differentiation from producing some IFNγ to secreting some potent anti-inflammatory IL-10, acquiring a so-called Type I regulatory phenotype (Tr1). Proteolytic cleavage of CD46 occurs upon costimulation and is important for T-cell activation and IL-10 production. The observation that CD46 cleavage was reduced when PBMCs were costimulated compared with purified CD4(+) T cells led us to hypothesize that interactions between different cell types within the PBMCs were able to modulate the CD46 pathway. We show that CD46 downregulation is also reduced when CD4(+) T cells are cocultured with autologous monocytes. Indeed, monocyte: T-cell cocultures impaired CD46-mediated T-cell differentiation and coactivation, by reducing downregulation of surface CD46, lowering induction of the early activation marker CD69, as well as reducing the levels of IL-...Continue Reading

References

Jun 8, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A AstierC Rabourdin-Combe
Dec 12, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·Y ZaffranA L Astier
Mar 26, 2002·Nature Reviews. Immunology·Arlene H Sharpe, Gordon J Freeman
Jan 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Edward M BertramTania H Watts
Jan 22, 2005·Immunology and Cell Biology·Andrea L DewarA Bruce Lyons
Jul 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jeffrey D PriceClaudia Kemper
Nov 14, 2006·The Journal of Clinical Investigation·Anne L AstierDavid A Hafler
Nov 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jane OliaroSarah M Russell
Jan 18, 2008·European Journal of Immunology·Ivan Martinez-ForeroPablo Villoslada
Jul 16, 2008·Immunology·Moa E FranssonAngelica S I Loskog
May 12, 2009·Immunological Reviews·Christopher E RuddHelga Schneider
Jun 10, 2010·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·Ya-Qing XuWei Guo
Jul 12, 2011·Immunobiology·Siobhan Ni Choileain, Anne L Astier
May 1, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Karoline KicklerAnne L Astier
Nov 13, 2012·PloS One·Karoline KicklerAnne L Astier
Feb 12, 2014·Proceedings of the National Academy of Sciences of the United States of America·Karen J MackenzieStephen M Anderton

❮ Previous
Next ❯

Citations

Apr 5, 2017·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Soobin ChungJi Youn Lee
Mar 3, 2018·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·D GençT Akkoç
May 3, 2019·Nature Reviews. Immunology·Edimara S ReisJohn D Lambris
Aug 29, 2018·Molecular Systems Biology·Daniela MichlmayrEva Harris
Apr 11, 2018·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Rosanna CoppoUNKNOWN VALIGA Study Group of the ERA-EDTA Immunonephrology Working Group
Aug 4, 2019·The AAPS Journal·Osman N YogurtcuHong Yang
Aug 27, 2017·Seminars in Immunopathology·Justin KillickAnne L Astier
Jun 6, 2018·Frontiers in Immunology·Laura A MichielsenHenny G Otten
Jun 9, 2017·Scientific Reports·Yuri AhnDo-Young Kim
Dec 15, 2020·Regenerative Engineering and Translational Medicine·Andrew D Fesnak
Jul 9, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rosario Luque-MartinPalwinder K Mander

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
flow cytometry
density gradient centrifugation
FACS

Software Mentioned

FlowJo

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved