Monodehydroascorbate reductase mediates TNT toxicity in plants

Science
Emily J JohnstonNeil C Bruce

Abstract

The explosive 2,4,6-trinitrotoluene (TNT) is a highly toxic and persistent environmental pollutant. Due to the scale of affected areas, one of the most cost-effective and environmentally friendly means of removing explosives pollution could be the use of plants. However, mechanisms of TNT phytotoxicity have been elusive. Here, we reveal that phytotoxicity is caused by reduction of TNT in the mitochondria, forming a nitro radical that reacts with atmospheric oxygen, generating reactive superoxide. The reaction is catalyzed by monodehydroascorbate reductase 6 (MDHAR6), with Arabidopsis deficient in MDHAR6 displaying enhanced TNT tolerance. This discovery will contribute toward the remediation of contaminated sites. Moreover, in an environment of increasing herbicide resistance, with a shortage in new herbicide classes, our findings reveal MDHAR6 as a valuable plant-specific target.

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Citations

Sep 5, 2015·Science·Graham Noctor
Sep 11, 2018·Plant, Cell & Environment·Vijay KumarKarl-Josef Dietz
May 23, 2020·Annual Review of Plant Biology·Christine H Foyer, Graham Noctor
Mar 9, 2017·The Biochemical Journal·Christine H FoyerGraham Noctor
Jul 20, 2017·Proceedings of the National Academy of Sciences of the United States of America·Carolyn Beans
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Oct 8, 2020·Journal, Genetic Engineering & Biotechnology·Siarhei A Dabravolski
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Nov 8, 2020·Journal of Proteomics·Ana Luiza S PaivaMárcia Margis-Pinheiro
Jun 14, 2021·Ecotoxicology and Environmental Safety·Jian-Jie GaoQuan-Hong Yao

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