PMID: 9427967Jan 15, 1998Paper

Monofilament intraluminal middle cerebral artery occlusion in the mouse

Neurological Research
W M ClarkF Eckenstein

Abstract

The rat middle cerebral artery (MCA) occlusion model with an intraluminal filament is well characterized with a two hour period of occlusion in widespread use. The recent availability of transgenic animals has led to an interest in adapting the MCA model in the mouse. To date the model has not been well characterized in the mouse. We performed the present study to compare different durations of MCA occlusion and to validate new functional assessments in this model. The MCA occlusion model (5-0 filament) was used. Swiss-Webster mice, 24-44 g, were randomly assigned to four groups: one hour of occlusion; two hours of occlusion; three hours of occlusion; or permanent occlusion. At 48 hours post-ischemia, the animals were rated on three neurologic function scales, and then the brains were removed for lesion size determination. Overall, there was a significant difference in lesion volume (p < 0.001) between the groups. In the permanent group of mice, the average lesion volume was 78.41 +/- 17.47 mm (n = 12); two and three hours of ischemia produced 51.29 +/- 29.82 mm3 (n = 11) and 54.85 mm3 (n = 13), respectively, significantly different than the one hour group 14.84 +/- 31.34 mm3 (n = 11). All three functional scoring systems found...Continue Reading

Citations

May 6, 1999·Nature Medicine·A SchneiderM Schwaninger
Aug 6, 2000·Stroke; a Journal of Cerebral Circulation·K HattoriA C DeVries
Apr 5, 2003·Acta Neurologica Scandinavica·M R Ardehali, G Rondouin
Sep 9, 2006·Stroke; a Journal of Cerebral Circulation·Xingshun XuBalvin H L Chua
Sep 18, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Shuzhen ChenYanfang Chen
Nov 20, 2014·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Fiona E BurrowsIngo Schiessl
Jul 18, 2017·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Anja KahlPing Zhou
Aug 10, 2005·Journal of Neurochemistry·Ralph AndreNancy J Rothwell
Jan 10, 2008·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Susan L StevensMary P Stenzel-Poore
May 18, 2006·The Journal of Pharmacology and Experimental Therapeutics·Claudio StoriniMaria Grazia De Simoni
Nov 23, 2006·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Carmen CaponeMaria Grazia De Simoni
Jan 15, 2008·Nature Protocols·Michal A RynkowskiE Sander Connolly
Jul 28, 2011·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Marc BredeChristoph Kleinschnitz
Mar 10, 2012·Stroke; a Journal of Cerebral Circulation·Philberta Y LeungMary P Stenzel-Poore
Jul 24, 2013·Stroke Research and Treatment·Fatima A Sehba, Ryszard M Pluta
Dec 15, 2015·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Lucy M TevesMichael Tymianski
May 30, 2017·Neural Regeneration Research·Hai-Feng MaoChu-Zu Wu
Sep 8, 2017·Scientific Reports·Jin-Hua GuCheng-Xin Gong
Sep 30, 2018·Metabolic Brain Disease·Dominic D QuintanaMohammad Alvi
Aug 16, 2019·Stroke; a Journal of Cerebral Circulation·Michael BieberDirk M Hermann
Nov 7, 2019·Immunity, Inflammation and Disease·Breanne Y FarrisEdwin C K Wan
Oct 4, 2003·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Susan L StevensMary P Stenzel-Poore
Oct 9, 2004·Stroke; a Journal of Cerebral Circulation·Giuseppe PignataroLucio Annunziato
Jul 8, 2000·Stroke; a Journal of Cerebral Circulation·W M ClarkF Eckenstein

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