PMID: 2125470Sep 18, 1990Paper

Monofunctional chorismate mutase from Bacillus subtilis: kinetic and 13C NMR studies on the interactions of the enzyme with its ligands

Biochemistry
J V GrayJ R Knowles

Abstract

The interaction of the monofunctional chorismate mutase from Bacillus subtilis with chorismate and prephenate has been studied kinetically and by NMR spectroscopy with 13C specifically labeled substrates. Prephenate dominates the population of enzyme-bound species, and the "off" rate constant (approximately 60 s-1) obtained from line-broadening experiments is close to the value of kcat for chorismate (50 s-1) determined kinetically. The calculated "on" rate constant for prephenate (8 x 10(5) M-1 s-1) is similar to the value of kcat/Km for chorismate (5 x 10(5) M-1 s-1). The kinetic parameters of the Bacillus mutase are remarkably insensitive to pH over a wide range and display no solvent isotope effect. These results suggest that the enzyme-catalyzed reaction may be encounter controlled (slowed from the diffusion limit by some feature of the enzyme's active site) and that kcat for chorismate is determined by the product off rate. There is now no evidence to suggest that the skeletal rearrangement on the enzyme surface occurs by a pathway other than a pericyclic process.

References

Sep 5, 1978·Biochemistry·H Görisch
Jun 7, 1979·Journal of Theoretical Biology·P R Andrews, E Heyde
Nov 28, 1989·Biochemistry·J J BurbaumJ R Knowles

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Citations

Oct 10, 2001·Angewandte Chemie·Sean V. TaylorDonald Hilvert
Dec 4, 2003·Journal of the American Society for Mass Spectrometry·Silke WendtRenato Zenobi
Apr 2, 1999·European Journal of Biochemistry·P MatteiD Hilvert
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y M ChookW N Lipscomb
Jan 1, 1997·Annual Review of Biophysics and Biomolecular Structure·H Wade, T S Scanlan
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·J MaW N Lipscomb
Aug 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·H GuoM Karplus
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·G SchnappaufG H Braus
Aug 31, 2000·Annual Review of Biochemistry·D Hilvert
Aug 6, 2008·Journal of Molecular Biology·Katherina VamvacaDonald Hilvert
Mar 29, 2000·Journal of Theoretical Biology·B MaR Nussinov
Aug 29, 2000·The Journal of Biological Chemistry·P KastD Hilvert
Nov 8, 2001·The Journal of Biological Chemistry·Joseph M Jez, Joseph P Noel
Sep 29, 1995·Science·P G Schultz, R A Lerner

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