Monoglycerides and diglycerides synthesis in a solvent-free system by lipase-catalyzed glycerolysis

Applied Biochemistry and Biotechnology
Patricia Bogalhos Lucente FregolenteRubens Maciel Filho

Abstract

Five lipases were screened (Thermomyces lanuginosus free and immobilized forms, Candida antarctica B, Candida rugosa, Aspergillus niger, and Rhizomucor miehei) to study their ability to produce monoglycerides (MG) and diglycerides (DG) through enzymatic glycerolysis of soybean oil. Lipase from C. antarctica was further studied to verify the enzyme load (wt% of oil mass), the molar ratio glycerol/oil, and the water content (wt% of glycerol) on the glycerolysis reaction. The best DG and MG productions were in the range 45-48% and 28-30% (w/w, based on the total oil), respectively. Using immobilized lipases, the amount of free fatty acids (FFA) produced was about 5%. However, the amount of FFA produced when using free lipases, with 3.5% extra water in the system, is equivalent to the MG yield, about 23%. The extra water content provides a competition between hydrolysis and glycerolysis reactions, increasing the FFA production.

Citations

Oct 29, 2009·Applied Biochemistry and Biotechnology·Patricia Bogalhos Lucente FregolenteRubens Maciel Filho
Oct 4, 2011·European Journal of Lipid Science and Technology : EJLST·Ning LiuMouming Zhao
Jul 14, 2016·Biotechnology and Applied Biochemistry·Juan DongRuijin Yang
Jan 11, 2020·Applied Biochemistry and Biotechnology·Nazanin VafaeiMartin G Scanlon
Apr 16, 2021·Current Research in Food Science·Reed A Nicholson, Alejandro G Marangoni

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