PMID: 7024083Jun 1, 1981Paper

Monoiodoinsulin labelled in tyrosine residue 16 or 26 of the insulin B-chain. Preparation and characterization of some binding properties

Hoppe-Seyler's Zeitschrift für physiologische Chemie
S LindeJ Gliemann

Abstract

By the combination of polyacrylamide gel electrophoresis and QAE-Sephadex ion exchange chromatography it was possible to isolate the four isomers of porcine [125I]monoiodoinsulin to a purity of more than 97%. The yield of the two B-chain-labelled isomers was increased by iodinating in buffer containing 6M urea. The apparent binding affinity to isolated rat adipocytes was 0.65 for the A19 isomer, 1.0 for the B16 isomer, and 2.0 for the B26 isomer, respectively, relative to the A14 isomer. In contrast, the B26 isomer had only an apparent binding affinity of 1.2 relative to the A14 isomer in isolated hepatocytes.

References

Dec 1, 1977·Experimental Cell Research·H PertoftB Klingeborn
Apr 27, 1979·Biochemical and Biophysical Research Communications·J GliemannB Hansen
Oct 27, 1975·Biochemical and Biophysical Research Communications·P De Meyts, J Roth
Jan 1, 1974·International Journal of Peptide and Protein Research·S Linde, B Hansen
May 1, 1980·International Journal of Peptide and Protein Research·S Linde, B Hansen

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Citations

Jun 9, 1983·Biochimica Et Biophysica Acta·J S BrushJ Gliemann
Mar 31, 1983·Biochimica Et Biophysica Acta·J GliemannL Sottrup-Jensen
Feb 25, 1983·Journal of Chromatography·B S WelinderJ S Brush
Sep 1, 1990·Analytical Biochemistry·A TuongM Gachon
Feb 15, 1986·Archives of Biochemistry and Biophysics·E Helmerhorst, G B Stokes
Apr 1, 1986·Diabetes Research and Clinical Practice·S M KelemenM K Gould
Oct 1, 1992·Analytical Biochemistry·D T De Leo, E Helmerhorst
May 16, 1988·Biochemical and Biophysical Research Communications·C F BurantM G Buse
Nov 23, 2016·The Journal of Biological Chemistry·Krystel El HageMichael A Weiss
Jul 6, 2014·The Journal of Biological Chemistry·Vijay PandyarajanMichael A Weiss
May 1, 1983·Hoppe-Seyler's Zeitschrift für physiologische Chemie·H J WienekeH Zahn
Mar 1, 1989·Biological Chemistry Hoppe-Seyler·F WedekindD Brandenburg
Jan 9, 2018·Structural Dynamics·Krystel El HageMarkus Meuwly
Aug 30, 1985·Journal of Chromatography·V Pingoud

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