PMID: 9426364Jan 14, 1998Paper

Monoisopropylglutathione ester protects A549 cells from the cytotoxic effects of sulphur mustard

Human & Experimental Toxicology
Christopher D LindsayA F Lailey

Abstract

1. The A549 cell line was used to assess the toxicity of sulphur mustard (HD), using gentian violet (GV) and neutral red (NR) dyes as indicators of cell viability. It was found that exposure to concentrations in excess of 40 microM HD resulted in a rapid onset of toxicity. 2. The ability of monoisopropylglutathione ester (MIPE) to protect A549 cells against the effects of a 100 microM challenge dose of HD was determined using the NR and GV assays. It was found that MIPE (8 mM) could protect cells against the effects of HD though MIPE had to be present at the time of HD challenge. Cultures protected with MIPE were two times more viable than HD exposed cells 48 h after HD challenge when using the GV and NR assays to assess viability. Observations by phase contrast microscopy of NR and GV stained cultures confirmed these findings. Addition of MIPE after previously exposing the A549 cultures to HD (for up to 5 min) maintained cell viability at 72% compared to 37% for unprotected cultures, after which time viability fell significantly so that at 10 min there was no difference in viability between the MIPE treated and untreated cultures. 3. Pretreating A549 cultures with MIPE for 1 h followed by its removal prior to HD challenge did ...Continue Reading

References

Dec 11, 1991·Biochemical Pharmacology·A F LaileyD G Upshall
Jan 1, 1990·Pharmacology & Therapeutics·E A Bump, J M Brown
Mar 15, 1989·Toxicology and Applied Pharmacology·M A MolA W Kluivers
Nov 15, 1986·Analytical Biochemistry·R J GilliesM Denton
Mar 1, 1980·Mutation Research·M Fox, D Scott
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·K AsanoJ S Stamler
Nov 1, 1994·Human & Experimental Toxicology·P E Wilde, D G Upshall
Jun 1, 1994·American Journal of Respiratory and Critical Care Medicine·X Y LiW MacNee
Jun 13, 1994·FEBS Letters·P VenembreG Durand
Jul 1, 1994·Experimental Cell Research·S L Sigurdson, J S Lwebuga-Mukasa
Apr 1, 1994·American Journal of Respiratory Cell and Molecular Biology·F WuD Warburton
Jan 1, 1996·Photochemistry and Photobiology·S KobayashiC Tohyama
Oct 1, 1963·The Biochemical Journal·P BROOKES, P D LAWLEY
Nov 1, 1948·The Journal of General Physiology·R M HERRIOTT
Mar 1, 1992·Toxicology in Vitro : an International Journal Published in Association with BIBRA·E GentilhommeJ Thivolet

❮ Previous
Next ❯

Citations

Nov 15, 2000·Toxicology and Applied Pharmacology·U WormserA Nyska
Mar 5, 2005·Journal of Applied Toxicology : JAT·Rachel Simpson, Christopher D Lindsay
Nov 22, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Alexander MargulisRami Neuman
Dec 29, 1998·Human & Experimental Toxicology·C D Lindsay, J L Hambrook
Mar 22, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Christophe GuignabertMarie-Pia d'Ortho
Oct 3, 2019·Critical Reviews in Toxicology·Leila EtemadMahdi Balali-Mood
Feb 13, 1999·Human & Experimental Toxicology·A AmirN Allon

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Pharmaceutical and Biomedical Analysis
Jawahar LalRam Chandra Gupta
Toxicology in Vitro : an International Journal Published in Association with BIBRA
T W Sawyer, M G Hamilton
© 2021 Meta ULC. All rights reserved