Monolayer and spheroid culture of human liver hepatocellular carcinoma cell line cells demonstrate distinct global gene expression patterns and functional phenotypes.

Tissue Engineering. Part a
Tammy T Chang, Millie Hughes-Fulford

Abstract

Understanding cell biology of three-dimensional (3D) biological structures is important for more complete appreciation of in vivo tissue function and advancing ex vivo organ engineering efforts. To elucidate how 3D structure may affect hepatocyte cellular responses, we compared global gene expression of human liver hepatocellular carcinoma cell line (HepG2) cells cultured as monolayers on tissue culture dishes (TCDs) or as spheroids within rotating wall vessel (RWV) bioreactors. HepG2 cells grown in RWVs form spheroids up to 100 mum in diameter within 72 h and up to 1 mm with long-term culture. The actin cytoskeleton in monolayer cells show stress fiber formation while spheroids have cortical actin organization. Global gene expression analysis demonstrates upregulation of structural genes such as extracellular matrix, cytoskeletal, and adhesion molecules in monolayers, whereas RWV spheroids show upregulation of metabolic and synthetic genes, suggesting functional differences. Indeed, liver-specific functions of cytochrome P450 activity and albumin production are higher in the spheroids. Enhanced liver functions require maintenance of 3D structure and environment, because transfer of spheroids to a TCD results in spheroid disint...Continue Reading

References

Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·F M WattC H O'Neill
Feb 1, 1987·The American Journal of Physiology·C E LloydL S Jefferson
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·C D RoskelleyM J Bissell
May 30, 1997·Science·C S ChenD E Ingber
Nov 22, 1997·Journal of Biomedical Materials Research·D J MooneyR Langer
Aug 13, 1998·Nature Medicine·B R Unsworth, P I Lelkes
Nov 5, 1999·In Vitro Cellular & Developmental Biology. Animal·V I KhaoustovB Yoffe
Aug 15, 2000·Tissue Engineering·S S KimJ P Vacanti
Jun 16, 2001·American Journal of Physiology. Renal Physiology·T G Hammond, J M Hammond
Sep 7, 2001·Advances in Space Research : the Official Journal of the Committee on Space Research (COSPAR)·B YoffeV I Khaoustov
Apr 8, 2004·Developmental Cell·Rowena McBeathChristopher S Chen
May 26, 2004·Transplant Immunology·Katherine M Kulig, Joseph P Vacanti
Sep 9, 2004·Science's STKE : Signal Transduction Knowledge Environment·Millie Hughes-Fulford
May 5, 2005·Tissue Engineering·Susan Fugett Abu-AbsiLinda K Hansen
Jul 27, 2005·Histochemistry and Cell Biology·Danko S BatusicGiuliano Ramadori
May 6, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Donald E Ingber
Jun 2, 2006·Cytokine & Growth Factor Reviews·Sassan Hafizi, Björn Dahlbäck
Jun 7, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Chai-Fei Li, Millie Hughes-Fulford
Nov 24, 2011·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons

❮ Previous
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Citations

Mar 25, 2010·In Vitro Cellular & Developmental Biology. Animal·Neil C TalbotLouis S Stodieck
Dec 9, 2010·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jianhua TangYinkun Liu
Nov 19, 2011·Journal of Laboratory Automation·David D NolteKwan Jeong
Apr 16, 2013·Nature Reviews. Cancer·Jeanne L Becker, Glauco R Souza
Sep 9, 2010·Biofabrication·Chang Mo HwangAli Khademhosseini
Apr 16, 2011·Tissue Engineering. Part B, Reviews·Melissa A KinneyTodd C McDevitt
Jul 30, 2010·Cold Spring Harbor Perspectives in Biology·Lyubomira Chakalova, Peter Fraser
Sep 8, 2011·Journal of Biomedical Optics·David D NolteKwan Jeong
Mar 7, 2014·Toxicon : Official Journal of the International Society on Toxinology·Amber F Roegner, Birgit Puschner
Mar 13, 2014·Advanced Drug Delivery Reviews·Anna Astashkina, David W Grainger
Jun 21, 2014·Future Oncology·Parul Benien, Archana Swami
Aug 15, 2014·Biotechnology Advances·Xian XuXinqiao Jia
Jul 16, 2014·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Can SarisozenVladimir P Torchilin
Mar 7, 2014·Tissue Engineering. Part B, Reviews·Daniela GrimmJohann Bauer
Mar 15, 2013·American Journal of Physiology. Cell Physiology·A DashB R Wamhoff
May 8, 2015·International Journal of Molecular Sciences·Sello Lebohang ManotoHeidi Abrahamse
May 29, 2012·Expert Opinion on Drug Metabolism & Toxicology·Ajit DashBrian R Wamhoff
Feb 28, 2013·Expert Opinion on Drug Delivery·Abraham H Abouzeid, Vladimir P Torchilin
Sep 10, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Bongseop KwakBumsoo Han
Jul 6, 2014·Advanced Drug Delivery Reviews·David T Leong, Kee Woei Ng
Jul 6, 2014·Advanced Drug Delivery Reviews·Gabriel BentonJennifer Koblinski
Aug 12, 2014·Advanced Drug Delivery Reviews·Salah-Eddine Lamhamedi-CherradiJoseph A Ludwig
Feb 10, 2016·Proceedings of the National Academy of Sciences of the United States of America·Xuanyi MaShaochen Chen
Oct 31, 2012·Colloids and Surfaces. B, Biointerfaces·M MassimiC Cametti
Mar 9, 2011·Biochemical and Biophysical Research Communications·Mahboobe GhaediAlexander Revzin
Dec 15, 2015·Methods : a Companion to Methods in Enzymology·Tracy A HookwayTodd C McDevitt
Apr 21, 2009·Biomaterials·Caroline N JonesAlexander Revzin
Dec 11, 2014·Materials Science & Engineering. C, Materials for Biological Applications·P R SarikaP R Anil Kumar

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Methods Mentioned

BETA
Assay
fluorescence microscopy
ELISA
enzyme-linked immunosorbent assay
GTPase

Software Mentioned

GeneSpring
InStat
Operon
Oligo

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