PMID: 9661053Jul 14, 1998Paper

Monolayer formation and DNA synthesis of the outer epithelial cells from pearl oyster mantle in coculture with amebocytes

In Vitro Cellular & Developmental Biology. Animal
M Awaji, T Suzuki

Abstract

In vitro experiments were conducted to clarify the involvement of the epithelium-amebocyte interaction in epithelial regeneration of bivalves. The outer epithelia of the pallial mantle of the pearl oyster. Pinctada fucata martensii, were separated in cell sheets from the inner connective tissue layers by digestion with Dispase. Clumps of the separated mantle epithelia were inoculated onto the amebocyte layers prepared on the bottom of culture dishes and maintained at 20 degrees C in 5% CO2:95% air for 1 wk. Balanced salt solution with 0.03% (wt/vol) glucose was used as a culture medium. The epithelial cells adhered to the amebocyte layers within 24 h, changed their shape from cuboidal to squamous, and migrated and formed monolayer sheets within 3 d. Electron microscopy confirmed maintenance of epithelial polarity and cell to cell junction in the sheets; 6 d after the inoculation, 5-bromo-2'-deoxyuridine was added to the culture at 30 microns. After labeling for 24 h, the cultures were fixed and stained with anti 5-bromo-2'-deoxyuridine antibody. Cells with immunoreactive nuclei were clearly observed in the epithelial cell sheets, indicating active DNA synthesis in the epithelial sheets. Thus, cocultured with amebocytes, the out...Continue Reading

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Citations

Aug 10, 2013·Cytotechnology·Matthew O'NeillStéphanie Auzoux-Bordenave
Nov 8, 2013·Canadian Journal of Zoology·T P YoshinoC J Bayne
Aug 19, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Ning WangShugo Watabe
Apr 1, 2009·Fish & Shellfish Immunology·N Gustaf F Mamangkey, Paul C Southgate
Feb 8, 2005·Tissue & Cell·C P Suja, S Dharmaraj
Feb 8, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Hisanori NozawaNobuo Seki
Jan 24, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Héctor Acosta-Salmón, Paul C Southgate
Jun 28, 2018·In Vitro Cellular & Developmental Biology. Animal·Vidya JayasankarIndira Divipala
Dec 7, 2018·Developmental and Comparative Immunology·José A Fernández RobledoMichael J Metzger

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