Monolayer-protected nanoparticle film assemblies as platforms for controlling interfacial and adsorption properties in protein monolayer electrochemistry

Journal of the American Chemical Society
Andrew F LoftusMichael C Leopold

Abstract

Assembled films of nonaqueous nanoparticles, known as monolayer-protected clusters (MPCs), are investigated as adsorption platforms in protein monolayer electrochemistry (PME), a strategy for studying the electron transfer (ET) of redox proteins. Modified electrodes featuring MPC films assembled with various linking methods, including both electrostatic and covalent mechanisms, are employed to immobilize cytochrome c (cyt c) for electrochemical analysis. The background signal (non-Faradaic current) of these systems is directly related to the structure and composition of the MPC films, including nanoparticle core size, protecting ligand properties, as well as the linking mechanism utilized during assembly. Dithiol-linked films of Au225(C6)75 are identified as optimal films for PME by sufficiently discriminating against detrimental background current and exhibiting interfacial properties that are readily engineered for cyt c adsorption and electroactivity (Faradaic current). Surface concentrations and denaturation rates of adsorbed cyt c are dictated by specific manipulation of the individual MPCs composing the outer layer of the film. The use of specially designed, hydrophilic MPCs as a terminal film layer results in near-ideal ...Continue Reading

References

Apr 27, 1977·Journal of the American Chemical Society·H L LandrumF M Hawkridge
Jun 1, 1991·Trends in Biotechnology·M Wahlgren, T Arnebrant
Jan 1, 1996·Annual Review of Biophysics and Biomolecular Structure·M Mrksich, G M Whitesides
Dec 10, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·S Oscarsson
Mar 31, 1998·Biosensors & Bioelectronics·A L GhindilisE Wilkins
Mar 29, 2000·Accounts of Chemical Research·A C TempletonR W Murray
Jul 26, 2002·Journal of the American Chemical Society·Francis P ZamboriniRoyce W Murray
Jul 16, 2003·Accounts of Chemical Research·Roy Shenhar, Vincent M Rotello
May 3, 2005·Analytical and Bioanalytical Chemistry·P Yáñez-Sedeño, J M Pingarrón
Nov 16, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·D J TognarelliMichael C Leopold
Feb 16, 2006·Analytical Chemistry·Rebecca L Wolfe, Royce W Murray
Mar 23, 2006·Journal of the American Chemical Society·Paul RoachCarole C Perry
Jun 14, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Prashanth AsuriJonathan S Dordick
Apr 28, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Koji NakanoEdmond F Bowden
Jun 15, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Lesley E RussellMichael C Leopold

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Citations

Mar 10, 2010·Analytical and Bioanalytical Chemistry·Jing ZhaoGenxi Li
Aug 9, 2008·Chemical Communications : Chem Comm·Sarita SitaulaScott M Reed
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Nadav AmdurskyDavid Cahen
Jun 7, 2017·Chemical Reviews·Indranath Chakraborty, Thalappil Pradeep
Aug 15, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Morgan L VargoMichael C Leopold
Mar 12, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Adrienne R SchmidtMichael C Leopold
Apr 14, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Blake N Johnson, Raj Mutharasan
Jan 7, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Nicholas G PoulosMichael C Leopold

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