Monolithic bioreactors: effect of chymotrypsin immobilization on its biocatalytic properties

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
E A PonomarevaT Tennikova

Abstract

The effect of different modes of alpha-chymotrypsin attachment to the surface of methacrylate-based ultrashort monolithic minicolumns on enzyme activity has been studied. The immobilization of protease was carried out via direct covalent binding of chymotrypsin, as well as via its attachment through small and polymer spacers. It was established that the lowest enzyme activity against N-benzoyl-l-tyrosine ethyl ester was found for bioreactor obtained via direct attachment of chymotrypsin to the surface of GMA-EDMA minidisks, whereas the highest parameter close to that determined for dissolved enzyme was found in the case of bioreactor prepared by the introduction of copolymer of 2-deoxy-N-methacryloylamido-d-glucose with N-vinylpyrrolidone and acrolein as a long and flexible polymer spacer. Additionally, the effect of flow rate of substrate recirculation on bioconversion efficiency was examined. Independently on immobilization method, the increase of flow rate led to the raise of biocatalytic efficiency.

References

Feb 15, 2003·Analytical Chemistry·Kumiko Sakai-KatoToshimasa Toyo'oka
Sep 6, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Martina VodopivecAles Strancar
Sep 18, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·C DelattreM A Vijayalakshmi

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Citations

May 31, 2012·Analytical and Bioanalytical Chemistry·Jens SproßAndrea Sinz
May 15, 2012·Analytical and Bioanalytical Chemistry·Bekir ÇelebiAli Tuncel
Sep 27, 2012·Analytical and Bioanalytical Chemistry·E A PonomarevaT B Tennikova
Feb 18, 2014·Journal of Chromatography. a·Aleš PodgornikNika Lendero Krajnc
Mar 8, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Aleš PodgornikNika Lendero Krajnc
Mar 23, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Laura Verónica CappellaGuillermo Picó
Jan 6, 2011·Journal of Chromatography. a·E F MaksimovaT B Tennikova
Jan 8, 2013·Journal of Separation Science·Evgenia G Vlakh, Tatiana B Tennikova
Mar 16, 2013·Journal of Separation Science·Evgenia G Vlakh, Tatiana B Tennikova
Aug 26, 2014·Journal of Separation Science·Tingting HongYibing Ji
Aug 24, 2017·Electrophoresis·Mariia V VolokitinaEvgenia G Korzhikova-Vlakh
Oct 19, 2013·Inorganic Chemistry·D V KrupenyaT B Tennikova

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