Monolithic capillary electrophoresis device with integrated fluorescence detector

Analytical Chemistry
J R WebsterC H Mastrangelo

Abstract

A monolithic capillary electrophoresis system with integrated on-chip fluorescence detector has been microfabricated on a silicon substrate. Photodiodes in the silicon substrate measure fluorescence emitted from eluting molecules. The device incorporates an on-chip thin-film interference filter that prevents excitation light from inhibiting the fluorescence detection. A transparent AZO conducting ground plane is also used to prevent the high electric fields used for the separation from interfering with the photodiode response. Separations of DNA restriction fragments have been performed in these devices with femtogram detection limits using SYBR Green I intercalating dye.

References

Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·A T Woolley, R A Mathies
Oct 15, 1995·Analytical Chemistry·A T Woolley, R A Mathies
Mar 10, 1998·Analytical Chemistry·A T WoolleyR A Mathies
Oct 17, 1998·Science·M A BurnsD T Burke
Jun 1, 1968·Applied Optics·R Fisher

❮ Previous
Next ❯

Citations

Sep 5, 2002·Electrophoresis·Wei-Lung TsengHuan-Tsung Chang
May 11, 2005·Analytical and Bioanalytical Chemistry·Kyo MiyakiKatsumi Uchiyama
Jun 24, 2006·Analytical and Bioanalytical Chemistry·Changqing YiMengsu Yang
Jun 21, 2005·Analytical and Bioanalytical Chemistry·S Büttgenbach, R Wilke
Jul 21, 2011·Analytical and Bioanalytical Chemistry·Mohana MarimuthuSanghyo Kim
Jun 9, 2005·Biomedical Microdevices·T KameiR A Mathies
Jun 22, 2010·Biomedical Microdevices·Arvind Chandrasekaran, Muthukumaran Packirisamy
Jan 27, 2004·Advanced Drug Delivery Reviews·S Zafar RazzackiMark A Burns
Nov 8, 2003·Journal of Chromatography. a·Evan ThrushJames S Harris
Aug 24, 2010·IET Nanobiotechnology·M BehnamC J Backhouse
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Wonhee LeeMichael L Roukes
Aug 17, 2011·International Journal of Molecular Sciences·Shohei KanedaTeruo Fujii
Jan 27, 2011·Biomicrofluidics·K OnoT Fujii
Nov 10, 2009·Journal of Biomedical Optics·Arvind Chandrasekaran, Muthukumaran Packirisamy
Nov 19, 2005·Journal of Biochemical and Biophysical Methods·Sudip Mondal, V Venkataraman
Sep 1, 2005·Medical Engineering & Physics·Xia XuZhanfeng Cui
Dec 24, 2005·Lab on a Chip·Bhaskar MitraYogesh B Gianchandani
Aug 13, 2004·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Victor M UgazMark A Burns
Sep 9, 2006·Electrophoresis·Swomitra K MohantyDavid J Beebe
Aug 24, 2007·Lab on a Chip·Dongliang YinHolger Schmidt
Jul 27, 2007·Lab on a Chip·Marc DandinElisabeth Smela
Dec 17, 2008·Electrophoresis·Dan XiaoMartin M F Choi
May 9, 2009·Electrophoresis·Swomitra K MohantyDavid J Beebe

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.