Monolithic column with double mixed-modes of hydrophilic interaction/cation-exchange and reverse-phase/cation-exchange stationary phase for pressurized capillary electrochromatography

Electrophoresis
Jiabin WangZenghong Xie

Abstract

A monolithic capillary column with double mixed-modes of hydrophilic interaction/cation-exchange and RP/cation-exchange stationary phase was prepared by in situ thermal polymerization and then hydrolyzed with hydrochloric acid. The polymerization solution containing glycidyl methacrylate (GMA), 3-sulfopropyl methacrylate potassium salt (SPMA), and ethylene dimethacrylate (EDMA) in a binary porogenic solvent consisting of dimethylformamide (DMF) and 1,4-butanediol was polymerized in a fused-silica capillary pretreated with 3-(trimetoxysilyl) propyl methacrylate. The epoxy groups on the surface were hydrolyzed to diol groups with hydrochloric acid to enhance the polarity of the stationary phase. By simply altering the ACN content in the mobile phase, two mixed-mode mechanisms could be achieved on the same monolithic column in different mobile phase condition. Hydrophilic interaction (or hydrophilic interaction/cation-exchange) was observed at high ACN content, as well as RP (or RP/cation-exchange) was observed at low ACN content. The monolithic column provided good selectivity and high efficiency for separation of neutral polar analytes and basic compounds. Phenols, anilines, alkaloids, nucleic acid bases, and narcotic pharmaceut...Continue Reading

References

Apr 28, 1999·Journal of Chromatography. a·T Helboe, S H Hansen
Jan 16, 2007·Journal of Biochemical and Biophysical Methods·Tohru IkegamiNobuo Tanaka

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Citations

Feb 12, 2009·Analytical and Bioanalytical Chemistry·Anna Nordborg, Emily F Hilder
Jun 17, 2010·Se pu = Chinese journal of chromatography·Xucong LinZenghong Xie
Jun 12, 2009·Electrophoresis·Frantisek Svec
Jul 3, 2013·Biomedical Chromatography : BMC·Szymon DziombaTomasz Bączek

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