Monolithic peptidyl sorbents for comparison of affinity properties of plasminogen activators

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Evgenia G VlakhTatiana B Tennikova

Abstract

Plasminogen activators are the proteases which convert plasminogen into plasmin dissolving, in its turn, the major component of blood clots, fibrin. They are extremely useful in heart attack therapy. Modern and most appropriate way of scaled up production of these valuable proteins is gene engineering. In this case, a separation and a purification of target product become the important steps of the whole process. Recently developed affinity chromatography on short monolithic columns seems to be a very attractive method for these purposes. High speed of a process prevents the protein's denaturation due to temperature or/and solvents influence. The better mass transfer mechanism (convection rather than diffusion) allows considering only biospecific complexing as time limiting step. Specificity of several synthetic peptides to plasminogen activators have been studied by affinity chromatography on short monolithic columns. Peptide ligands were synthesized by conventional solid phase peptide synthesis (SPPS). The immobilization procedure was carried out as a one step process at static conditions. The results of quantitative evaluation of such affinity interactions were compared with those established for plasminogen that is the natu...Continue Reading

References

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Oct 29, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·R NecinaA Jungbauer
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Mar 29, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·D JosicA Jungbauer
Jan 16, 2002·Journal of Combinatorial Chemistry·Karin PflegerlAlois Jungbauer
May 10, 2002·Journal of Chromatography. a·Natalia D OstryaninaTatiana B Tennikova
Sep 5, 2002·Biotechnology and Bioengineering·K PflegerlA Jungbauer
Jan 7, 2005·Journal of Peptide Science : an Official Publication of the European Peptide Society·E VlakhT Tennikova

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Citations

Sep 15, 2006·Journal of Separation Science·Rangan Mallik, David S Hage
Mar 24, 2005·Journal of Chromatography. a·Galina A Platonova, Tatiana B Tennikova
Mar 24, 2005·Journal of Chromatography. a·Tatiana B Tennikova, Joachim Reusch

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