Monolithically-integrated cytometer for measuring particle diameter in the extracellular vesicle size range using multi-angle scattering.

Lab on a Chip
Jonathan T ButementJames S Wilkinson

Abstract

Size measurement of extracellular vesicles is hampered by the high cost and measurement uncertainty of conventional flow cytometers which is mainly due to the use of non-specialised free space optics. Integrated cytometry, where the optics and fluidics are embedded in a monolithic chip shows promise for the production of low cost, micro-flow cytometers dedicated for extracellular vesicle (EV) analysis with improved size measurement accuracy and precision. This research demonstrates a unique integrated cytometer for sub-micron particle size measurement using multi-angle scattering analysis. A combination of three technologies is used: (i) Dean-based hydrodynamic focussing to deliver a tight sample core stream to the analysis region, (ii) integrated waveguides with multimode interference devices to focus a narrow excitation beam onto the sample stream, and (iii) an angular array of collection waveguides to measure particle scattering distribution and calculate diameter. Low index 200 nm liposomes could be detected and polystyrene size standards as small as 400 nm diameter could be measured with an uncertainty of ±21 nm (1/2 IQR) demonstrating a first step on the path to high performance integrated cytometry of EVs.

References

May 24, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Rebecca A DragovicIan L Sargent
Aug 19, 2011·Optics Letters·Hamish C Hunt, James S Wilkinson
Mar 8, 2012·Journal of Thrombosis and Haemostasis : JTH·E van der PolT G van Leeuwen
Feb 19, 2014·Nature Reviews. Rheumatology·Edit I BuzasSteffen Gay
Mar 13, 2014·Journal of Thrombosis and Haemostasis : JTH·N ArraudA R Brisson
Jun 11, 2014·Lab on a Chip·Hamed AminiDino Di Carlo
Mar 27, 2015·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Anastasiya I KonokhovaValeri P Maltsev
Nov 14, 2015·Journal of Thrombosis and Haemostasis : JTH·Edwin van der PolRienk Nieuwland
Jan 9, 2016·The Journal of Endocrinology·Charlotte LawsonSean M Davidson
Aug 9, 2016·Biochimica Et Biophysica Acta. General Subjects·Déborah L M RupertMarta Bally
Sep 16, 2017·Frontiers in Cell and Developmental Biology·Joshua A WelshNicola A Englyst
Feb 1, 2018·Chemical Reviews·Huilin ShaoHakho Lee
Mar 27, 2018·Journal of Thrombosis and Haemostasis : JTH·E van der PolUNKNOWN ISTH-SSC-VB Working group
Apr 1, 2018·Journal of Leukocyte Biology·Joshua A WelshChristopher D Byrne
Jun 30, 2019·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Joshua A WelshNicola A Englyst

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
sizing
chips
chip
biopsy
fluorescence microscopy
fluorescence imaging
confocal microscopy

Software Mentioned

RSoft Synopsys
Beamprop
MATLAB®
Huygens Essential

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.

Related Papers

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Harald B Steen
Frontiers in Cell and Developmental Biology
Joshua A WelshNicola A Englyst
Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Joanne LanniganRobert Zucker
© 2021 Meta ULC. All rights reserved