Monomeric L-amino acid oxidase-induced mitochondrial dysfunction in Rhizoctonia solani Reveals a novel antagonistic mechanism of Trichoderma harzianum ETS 323

Journal of Agricultural and Food Chemistry
Chia-Ann YangKou-Cheng Peng

Abstract

The monomeric L-amino acid oxidase (mTh-LAAO) of Trichoderma harzianum ETS 323 has been suggested to antagonize Rhizoctonia solani by an unknown mechanism. Here, the mTh-LAAO-treated R. solani exhibited hyphal lysis and apoptotic characteristics such as DNA fragmentation, reactive oxygen species (ROS) accumulation, lipid peroxidation, and mitochondrial membrane potential depolarization. This hyphal lysis was suppressed by the mitochondria-dependent apoptosis inhibitor oligomycin while accompanied by reduction of ROS accumulation. This result suggested that mitochondria-mediated apoptosis in R. solani was involved in mTh-LAAO-induced growth inhibition, which was supported by the evidence of cytocheome c release and activation of caspases 9 and 3. Furthermore, the data indicated that the mTh-LAAO-induced fungal cell death was also closely interrelated with the interaction of mTh-LAAO with R. solani hyphal cell wall proteins. These results illuminate the biological function and mechanism underlying the antagonistic action of T. harzianum mTh-LAAO against fungal pathogens.

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Citations

Jan 31, 2014·Journal of Agricultural and Food Chemistry·Marthandam Asokan ShibuKou-Cheng Peng
Dec 20, 2013·Applied Microbiology and Biotechnology·Gazi Sakir HossainJian Chen
Jan 26, 2021·Plant Science : an International Journal of Experimental Plant Biology·Kou-Cheng PengKuo-Chih Lin
Mar 11, 2017·Microbiology Spectrum·Magnus KarlssonSusanne Zeilinger

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